Glucomannan Assay Kit

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

Product Code
50 Assays per kit

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UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

(1) Ac-Glucomannan + H2O → Ac-glucomanno-oligomers

                                                    (pH 12.5)
(2) Ac-Glucomanno-oligomers + H2O → glucomanno-oligomers
                                                                                 + acetate

                               (β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H2O → D-glucose + D-mannose

(4) D-glucose / D-mannose + ATP → G-6-P / M-6-P + ADP

      (glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

      (phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P               →              F-6-P               →               G-6-P

Kit size:                              50 assays
Method:                             Spectrophotometric at 340 nm
Total assay time:              120 min
Detection limit:                 1-100% of sample weight
Application examples:
Jelly sweets, cosmetics, food gums and other materials
Method recognition:       Novel method


  • Very cost effective
  • Only enzymatic kit available
  • Simple format
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Chemical composition and physicochemical properties of tubera salep produced from some Orchidaceae species.

Tekinşen, K. K. & Güner, A. (2010). Food Chemistry, 121(2), 468-471.

Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense.

Hsieh, Y. S. -Y., Chien, C., Liao, S. K. -S., Liao, S. F., Hung, W. T., Yang, W. B., Lin, C. C., Cheng, T. J. R.,Chang, C. C., Fanga, J. M. & Wong, C. H. (2008). Bioorganic & Medicinal Chemistry, 16(11), 6054-6068.

Methodologies for the extraction and analysis of konjac glucomannan from corms of Amorphophallus konjac K. Koch.

Chua, M., Chan, K., Hocking, T. J., Williams, P. A., Perry, C. J. & Baldwin, T. C. (2012). Carbohydrate Polymers, 87(3), 2202-2210.