Glucomannan Assay Kit

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

Product Code
Content/size
Stock
Price
Qty
K-GLUM
50 Assays per kit
$352.00

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UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

Principle:
                                    (β-mannanase)
(1) Ac-Glucomannan + H2O → Ac-glucomanno-oligomers

                                                    (pH 12.5)
(2) Ac-Glucomanno-oligomers + H2O → glucomanno-oligomers
                                                                                 + acetate


                               (β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H2O → D-glucose + D-mannose

                                                (hexokinase)
(4) D-glucose / D-mannose + ATP → G-6-P / M-6-P + ADP

      (glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

      (phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P               →              F-6-P               →               G-6-P

Kit size:                              50 assays
Method:                             Spectrophotometric at 340 nm
Total assay time:              120 min
Detection limit:                 1-100% of sample weight
Application examples:
Jelly sweets, cosmetics, food gums and other materials
Method recognition:       Novel method

Advantages

  • Very cost effective
     
  • Only enzymatic kit available
     
  • Simple format
     
  • All reagents stable for > 2 years after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

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Measurement of carbohydrates in grain, feed and food.

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Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense.

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Methodologies for the extraction and analysis of konjac glucomannan from corms of Amorphophallus konjac K. Koch.

Chua, M., Chan, K., Hocking, T. J., Williams, P. A., Perry, C. J. & Baldwin, T. C. (2012). Carbohydrate Polymers, 87(3), 2202-2210.

Rheological properties and structural characterization of salep improved by ethanol treatment.

Kurt, A. & Kahyaoglu, T. (2015). Carbohydrate polymers, 133, 654-661.