Glutaminase (E. coli

High purity recombinant Glutaminase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAS: 9001-47-2

glutaminase; L-glutamine amidohydrolase

Recombinant. From Escherichia coli.
In 2.5 M lithium sulphate.
Supplied at ~ 1,250 U/mL. 

Specific activity:
~ 515 U/mg (25oC, pH 4.9 on L-glutamine).

Stability: Minimum 1 year at 4oC. Check vial for details.

Product Code
2,500 Units

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Glutaminase (Escherichia coli)

CAS: 9001-47-2

glutaminase; L-glutamine amidohydrolase

In 2.5 M lithium sulphate.

Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 515 U/mg (25oC, pH 4.9 on L-glutamine).

Unit definition:
One Unit of glutaminase is defined as the amount of enzyme required to deaminate one μmole of L-glutamine (40 mM) to L-glutamate + NH4+ in sodium acetate buffer (40 mM) pH 4.9.

Catalyses the reaction:
Glutamine + H2O = Glutamate + NH3

Applications for the measurement of glutamine in the food, fermentation and clinical chemistry industries.

Construction of chiral ligand exchange capillary electrochromatography for D, L-amino acids enantioseparation and its application in glutaminase kinetics study.

Zhao, L., Qiao, J., Zhang, K., Li, D., Zhang, H. & Qi, L. (2018). Journal of Chromatography A, In Press.

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