Glycerol GK Assay Kit

The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
Content/size
Stock
Price
Qty
K-GCROLGK
70 assays (manual) / 700 assays (microplate)
/ 600 assays (auto-analyser)
$148.00

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UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
                     (glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

                           (ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP

       (glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD+ → gluconate-6-phosphate + NADH + H+

Kit size:    70 assays (manual) / 700 (microplate)
                                          / 600 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 7 min
Detection limit:                 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:        Novel method

Advantages

  • Novel tablet format for increased stability
     
  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years as supplied
     
  • Very rapid reaction
     
  • Positive reaction (assay proceeds with an increase in absorbance)
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Light enhanced calcification in Stylophora pistillata: effects of glucose, glycerol and oxygen.

Holcomb, M., Tambutté, E., Allemand, D. & Tambutté, S. (2014). PeerJ, 2, e375.

Dynamic modeling of methylotrophic Pichia pastoris culture with exhaust gas analysis: From cellular metabolism to process simulation.

Niu, H., Daukandt, M., Rodriguez, C., Fickers, P. & Bogaerts, P. (2013). Chemical Engineering Science, 87, 381-392.

Construction of self-cloning, indigenous wine strains of Saccharomyces cerevisiae with enhanced glycerol and glutathione production.

Hao, R. Y., Liu, Y. L., Wang, Z. Y. & Zhang, B. R. (2012). Biotechnology Letters, 34(9), 1711-1717.

Construction of an efficient xylose-fermenting diploid Saccharomyces cerevisiae strain through mating of two engineered haploid strains capable of xylose assimilation.

Kim, S. R., Lee, K. S., Kong, I. I., Lesmana, A., Lee, W. H., Seo, J. H., Kweon, D. H. & Jin, Y. S. (2013). Journal of Biotechnology, 164(1), 105-111.
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs