Hexokinase/Glucose-6-phosphate dehydrogenase

High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.7.1.1 (Hexokinase)
CAS: 9001-51-8

hexokinase; ATP:D-hexose 6-phosphotransferase

EC 1.1.1.49 (Glucose-6-phosphate dehydrogenase)
CAS: 9001-40-5

glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

Hexokinase: Recombinant. From yeast.
G6P-DH: Recombinant. From Leuconostoc mesenteroides.
In 3.2 M ammonium sulphate.

Activity/Concentration:
Hexokinase: ~ 420 U/mL.
G6P-DH: ~ 210 U/mL.

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-HKGDH
10 mL Hexokinase (420 U/ml) +
G6P-DH (210 U/ml)
$184.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

Hexokinase/Glucose-6-phosphate dehydrogenase

EC 2.7.1.1 (Hexokinase)
CAS: 9001-51-8

EC 1.1.1.49 (Glucose-6-phosphate dehydrogenase)
CAS: 9001-40-5

Synonyms:
(Hexokinase): Hexokinase; ATP:D-hexose 6-phosphotransferase
(G6P-DH): glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Activity/Concentration
Hexokinase: ~ 420 U/mL.
G6P-DH: ~ 210 U/mL.

Unit definition:
One Unit of hexokinase activity is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25oC. 

One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.4 and 25oC. 

Specificity:
Hexokinase:
Catalyses the reaction:
ATP + D-hexose = ADP + D-hexose 6-phosphate

Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Glucose-6-phosphate dehydrogenase:
Catalyses the reaction:
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+

Applications:
Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.

Simple purification method for a recombinantly expressed native His-tag-free aminopeptidase A from Lactobacillus delbrueckii.

Stressler, T., Tanzer, C., Ewert, J., Claaßen, W. & Fischer, L. (2017). Protein Expression and Purification, 131, 7-15.

A novel glutamyl (aspartyl)-specific aminopeptidase A from Lactobacillus delbrueckii with promising properties for application.

Stressler, T., Ewert, J., Merz, M., Funk, J., Claaßen, W., Lutz-Wahl, S., Schmidt, H., Kuhn, A. & Fischer, L. (2016). PloS one, 11(3), e0152139.

A fusion protein consisting of the exopeptidases PepN and PepX - production, characterization, and application.

Stressler, T., Pfahler, N., Merz, M., Hubschneider, L., Lutz-Wahl, S. Claaßen, W., & Fischer, L. (2016). Applied Microbiology and Biotechnology, 1-17, 7499-7515.

During yeast chronological aging resveratrol supplementation results in a short-lived phenotype Sir2-dependent.

Orlandi, I., Stamerra, G., Strippoli, M. & Vai, M. (2017). Redox Biology, 12, 745-754.