Hexokinase (yeast)
High purity Hexokinase (yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 2.7.1.1
CAS: 9001-51-8
hexokinase; ATP:D-hexose 6-phosphotransferase
Highly purified. From yeast.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,280 U/mL.
Specific activity:
~ 100 U/mg (25oC, pH 7.4 on glucose).
Stability: > 4 years at 4oC.
DESCRIPTION
Hexokinase (yeast)
EC 2.7.1.1
CAS: 9001-51-8
Synonyms:
hexokinase; ATP:D-hexose 6-phosphotransferase
Form:
In 3.2 M ammonium sulphate.
Stability:
> 4 years at 4oC.
Specific activity:
~ 100 U/mg (25oC, pH 7.4 on glucose).
Unit definition:
One Unit of hexokinase activity is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25oC.
Specificity:
Catalyses the reaction:
ATP + D-hexose = ADP + D-hexose 6-phosphate
Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.
Applications:
Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
