Hexokinase (yeast) 

High purity Hexokinase (yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.7.1.1
CAS: 9001-51-8

hexokinase; ATP:D-hexose 6-phosphotransferase

Highly purified. From yeast.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,280 U/mL. 

Specific activity:
~ 100 U/mg (25oC, pH 7.4 on glucose).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-HEX10
10,000 Units
$159.00

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DESCRIPTION

Hexokinase (yeast)

EC 2.7.1.1
CAS: 9001-51-8

Synonyms:
hexokinase; ATP:D-hexose 6-phosphotransferase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 100 U/mg (25oC, pH 7.4 on glucose).

Unit definition:
One Unit of hexokinase activity is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25oC.  

Specificity:
Catalyses the reaction:
ATP + D-hexose = ADP + D-hexose 6-phosphate

Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine.

Applications:
Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.