Hyaluronate lyase (novel specificity)
(soil prokaryote) 

High purity recombinant Hyaluronate lyase (novel specificity) (soil prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: PL8
CAS: 37259-53-3 

hyaluronate lyase

Recombinant. From a soil prokaryote.
In 3.2 M ammonium sulphate.
Supplied at ~ 100 U/mL. 

Specific activity:
~ 100 U/mg (25oC, pH 4.2 on hyaluronic acid).

Stability: > 4 years at 4oC. 

Product Code
100 Units

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Hyaluronate lyase (novel specificity) (soil prokaryote)

CAZy Family: PL8
CAS: 37259-53-3

hyaluronate lyase

In 3.2 M Ammonium sulphate.

> 4 years at 4oC.

Specific activity:
~ 100 U/mg (25oC, pH 4.2 on hyaluronic acid).

Unit definition:.
One Unit of hyaluronate lyase activity is defined as the amount of enzyme required to release one μmole of 4,5-unsaturated product per minute from hyaluronic acid (0.58 mM) in the presence of calcium chloride (0.1mM) in sodium succinate buffer (20 mM) pH 4.2 at 25oC.

Cleaves hyaluronate chains at a β-D-GalNAc-(1,4)-β-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine. 

Applications in the medical and pharmaceutical industries.