Invertase (fructofuranosidase) (yeast)

High purity Invertase (β-Fructofuranosidase) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.26
CAZy Family: GH43
CAS: 9001-57-4

beta-fructofuranosidase; beta-D-fructofuranoside fructohydrolase

Chromatographically purified. From yeast.
In 50% (v/v) glycerol.
Supplied at ~ 2,000 U/mL.

Specific activity
~ 300 U/mg protein (40oC, pH 4.5 on sucrose).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-INVRT
100 mL; 200,000 Units. 2,000 U/mL
$182.00

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DESCRIPTION

Invertase (fructofuranosidase) (yeast)

EC 3.2.1.26
CAZy Family: GH43

CAS: 9001-57-4

Synonyms:
beta-fructofuranosidase; beta-D-fructofuranoside fructohydrolase

Form:
In 50% (v/v) glycerol.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 300 U/mg (40oC, pH 4.5 on sucrose).

Unit definition:
One Unit of invertase activity is defined as the amount of enzyme required to hydrolyze one μmole of sucrose (1% w/v) per minute in sodium acetate buffer (100mM), pH 4.5 at 40oC.

Specificity:
Hydrolysis of terminal non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Applications:
Applications for the measurement of fructans in foods.

Method for the direct determination of available carbohydrates in low-carbohydrate products using high-performance anion exchange chromatography.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.

Efficacy of reducing sugar and phenol–sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs.

Hall, M. B. (2013). Animal Feed Science and Technology, 185(1), 94-100.

Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation.

Patton, A. J., Cunningham, S. M., Volenec, J. J. & Reicher, Z. J. (2007). Crop Science, 47(5), 2170-2181.