Isoamylase (Glycogen 6-glucanohydrolase) 

High purity Isoamylase (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6 

isoamylase; glycogen 6-alpha-D-glucanohydrolase

Highly purified. From Pseudomonas sp. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.
E-ISAMY-200U: Supplied at ~ 500 U/mL.
E-ISAMY-500U: Supplied at ~ 500 U/mL. 

Specific activity: 
~ 280 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).

Stability: > 4 years at 4oC. 

Data booklets for each pack size are located in the Documentation tab.

Product Code
Content/Size
Stock
Price
Qty
E-ISAMY-500U
500 units
$221.00
E-ISAMY-200U
200 units
$130.00

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DESCRIPTION

Isoamylase (Glycogen 6-glucanohydrolase)

EC 3.2.1.68 
CAZy Family: GH13
CAS: 9067-73-6 

Synonyms:
isoamylase; glycogen 6-alpha-D-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 280 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).

Unit definition:
One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Applications:
Applications in carbohydrate research and in the food and feeds, and cereals industry.

Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination.

Xia, H., Yandeau-Nelson, M., Thompson, D. B. & Guiltinan, M. J. (2011). BMC Plant Biology, 11(1), 95-107.

Effect of a gibberellin-biosynthesis inhibitor treatment on the physicochemical properties of sorghum starch.

Li, E., Hasjim, J., Dhital, S., Godwin, I. D. & Gilbert, R. G. (2011). Journal of Cereal Science, 53(3), 328-334.

Characterization of starch granules in rice culms for application of rice straw as a feedstock for saccharification.

Matsuki, J., Park, J. Y., Shiroma, R., Arai-Sanoh, Y., Ida, M., Kondo, M., Motobayashi, K. & Tokuyasu, K. (2010). Bioscience, Biotechnology, and Biochemistry, 74(8), 1645-1651.

Physico-chemical properties of potato starches.

Alvani, K., Qi, X., Tester, R. F. & Snape, C. E. (2011). Food Chemistry, 125(3), 958-965.

Determination of polydextrose as dietary fiber in foods.

Craig, S. A. S., Holden, J. F. & Khaled, M. Y. (2000). Journal of AOAC International, 83(4), 1006-1012.

Amylolysis of wheat starches. II. Degradation patterns of native starch granules with varying functional properties.

Blazek, J. & Copeland, L. (2010). Journal of Cereal Science, 52(2), 295-302.

Differences in structures of starch hydrolysates using saliva from different individuals.

Nantanga, K. K. M., Chan, E., Suleman, S., Bertoft, E. & Seetharaman, K. (2013). Starch‐Stärke, 65(7‐8), 709-713.

Determination of polydextrose in foods by ion chromatography: collaborative study.

Craig, S. A. S., Holden, J. F. & Khaled, M. Y. (2001). Journal of AOAC International, 84(2), 472-478.

Structure and function of starch and resistant starch from corn with different doses of mutant amylose-extender and floury-1 alleles.

Yao, N., Paez, A. V. & White, P. J. (2009). Journal of Agricultural and Food Chemistry, 57(5), 2040-2048.

Molecular structure and granule morphology of native and heat‐moisture‐treated pinhão starch.

Pinto, V. Z., Moomand, K., Vanier, N. L., Colussi, R., Villanova, F. A., Zavareze, E. R., Lim, L. T. & Dias, A. R. G. (2015). International Journal of Food Science & Technology, 50(2), 282-289.

An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment.

Vester, J. K., Glaring, M. A. & Stougaard, P. (2015). Applied Microbiology and Biotechnology, 99(2), 717-727.

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FAQs