Isocitrate dehydrogenase (Bacillus subtilis

High purity recombinant Isocitrate dehydrogenase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 1.1.1.42
CAS: 9028-48-2

isocitrate dehydrogenase (NADP+); isocitrate:NADP+ oxidoreductase (decarboxylating)

Recombinant. From Bacillus Subtilis.
In 3.2 M ammonium sulphate.
Supplied at ~ 380 U/mL. 

Specific activity:
~ 25 U/mg (25oC, pH 7.6 on D-isocitric acid).

Stability: Minimum 1 year at 4oC. Check vial for details.

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Content/size
Stock
Price
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E-ICDHBS
2,000 Units
 

DESCRIPTION

Isocitrate dehydrogenase (Bacillus subtilis)

EC 1.1.1.42
CAS: 9028-48-2

Synonyms:
isocitrate dehydrogenase (NADP+); isocitrate:NADP+ oxidoreductase (decarboxylating)

Form:
In 3.2 M ammonium sulphate.

Stability: 
Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 25 U/mg (25oC, pH 7.6 on D-isocitric acid).

Unit definition:
One unit of Isocitrate dehydrogenase is the amount of enzyme required to convert one μmole of D-/L-isocitric acid to α-ketoglutarate per minute in Tris.HCl buffer (143 mM), pH 7.6 at 25oC.

Specificity:
Catalyses the reaction:
Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+

Applications:
Applications for the measurement of isocitric acid in the food, fermentation and beverage industries.