L-Arabinose/D-Galactose Assay Kit

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products. 

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC. 

Suitable for manual, auto-analyser and microplate formats.

Product Code
Content/size
Stock
Price
Qty
K-ARGA
115 assays (manual) / 1150 assays (microplate)
/ 1150 assays (auto-analyser)
$268.00

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UV-method for the determination of L-Arabinose and D-Galactose
in hydrolysed plant products

Principle:
                                      (galactose mutarotase)
(1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose

                      (β-galactose dehydrogenase)
(2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+
                     
                    (β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                            * 115 assays (manual) / 1150 (microplate)
                                          / 1150 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 12 min (L-arabinose)
~ 6 min (D-galactose)
Detection limit:                 0.58 mg/L (L-arabinose)
0.69 mg/L (D-galactose)
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan,
arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods
containing milk (e.g. dietetic foods, bakery products, baby food,
chocolate, sweets and ice-cream), food additives (e.g. sweeteners),
cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:       Novel method

Advantages

  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Only enzymatic kit available
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

Novel bifunctional α-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum.

Ravanal, M. C., Callegari, E. & Eyzaguirre, J. (2010). Applied and Environmental Microbiology, 76(15), 5247-5253.

The influence of Aspergillus niger transcription factors AraR and XlnR in the gene expression during growth in D-xylose, L-arabinose and steam-exploded sugarcane bagasse.

de Souza, W. R., Maitan-Alfenas, G. P., de Gouvêa, P. F., Brown, N. A., Savoldi, M., Battaglia, E., Goldman M. H. S., deVries, R.P. & Goldman, G. H. (2013). Fungal Genetics and Biology, 60, 29-45.

Homologous expression and biochemical characterization of the arylsulfatase from Kluyveromyces lactis and its relevance in milk processing.

Stressler, T., Leisibach, D., Lutz-Wahl, S., Kuhn, A. & Fischer, L. (2016). Applied Microbiology and Biotechnology, 100(12), 5401-5414.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs