L-Arabinose/D-Galactose Assay Kit

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products. 

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC. 

Suitable for manual, auto-analyser and microplate formats.

Product Code
115 assays (manual) / 1150 assays (microplate)
/ 1150 assays (auto-analyser)

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UV-method for the determination of L-Arabinose and D-Galactose
in hydrolysed plant products

                                      (galactose mutarotase)
(1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose

                      (β-galactose dehydrogenase)
(2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+
                    (β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                            * 115 assays (manual) / 1150 (microplate)
                                          / 1150 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 12 min (L-arabinose)
~ 6 min (D-galactose)
Detection limit:                 0.58 mg/L (L-arabinose)
0.69 mg/L (D-galactose)
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan,
arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods
containing milk (e.g. dietetic foods, bakery products, baby food,
chocolate, sweets and ice-cream), food additives (e.g. sweeteners),
cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:       Novel method


  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

Novel bifunctional α-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum.

Ravanal, M. C., Callegari, E. & Eyzaguirre, J. (2010). Applied and Environmental Microbiology, 76(15), 5247-5253.

The influence of Aspergillus niger transcription factors AraR and XlnR in the gene expression during growth in D-xylose, L-arabinose and steam-exploded sugarcane bagasse.

de Souza, W. R., Maitan-Alfenas, G. P., de Gouvêa, P. F., Brown, N. A., Savoldi, M., Battaglia, E., Goldman M. H. S., deVries, R.P. & Goldman, G. H. (2013). Fungal Genetics and Biology, 60, 29-45.

Homologous expression and biochemical characterization of the arylsulfatase from Kluyveromyces lactis and its relevance in milk processing.

Stressler, T., Leisibach, D., Lutz-Wahl, S., Kuhn, A. & Fischer, L. (2016). Applied Microbiology and Biotechnology, 100(12), 5401-5414.

Penicillium purpurogenum produces a novel endo-1,5-arabinanase, active on debranched arabinan, short arabinooligosaccharides and on the artificial substrate p-nitrophenyl arabinofuranoside.

Vilches, F., Ravanal, M. C., Bravo-Moraga, F., Gonzalez-Nilo, D. & Eyzaguirre, J. (2018). Carbohydrate Research, 455, 106-113.

Mechanisms of utilisation of arabinoxylans by a porcine faecal inoculum: competition and co-operation.

Feng, G., Flanagan, B. M., Mikkelsen, D., Williams, B. A., Yu, W., Gilbert, R. G. & Gidley, M. J. (2018). Scientific Reports, 8(1), 4546.

Yeast lipids from cardoon stalks, stranded driftwood and olive tree pruning residues as possible extra sources of oils for producing biofuels and biochemicals.

Tasselli, G., Filippucci, S., Borsella, E., D’Antonio, S., Gelosia, M., Cavalaglio, G., Turchetti, B., Sannino, C., Onofri, A., Mastrolitti, S., De Bari, I., Cotana, F. & Bari, I. (2018). Biotechnology for Biofuels, 11(1), 147.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.