L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Product Code
Content/size
Stock
Price
Qty
K-ASNAM
100 assays (50 of each) per kit
$250.00

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UV-method for the determination of L-Asparagine, L-Glutamine
and Ammonia in potatoes, foodstuffs and cell culture media

Principle:
                               (glutaminase)
(1) L-Glutamine + H2O → L-glutamate + NH4+

                             (microbial glutamate dehydrogenase)
(2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

                                (asparaginase)
(3) L-Asparagine + H2O → L-aspartate + NH4+

Kit size:                              50 assays of each
Method:                              Spectrophotometric at 340 nm
Reaction time:                   ~ 20 min
Detection limit:                  0.50 mg/L (L-asparagine)
                                            0.54 mg/L (L-glutamine)
                                            0.06 mg/L (ammonia)
Application examples:
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:          Novel method

Advantages

  • Very rapid reaction due to use of uninhibited glutamate dehydrogenase
     
  • All enzymes supplied as stabilised suspensions
     
  • Only kit available
     
  • Very cost effective
     
  • All reagents stable for > 2 years after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

Oncolytic measles viruses produced at different scales under serum‐free conditions.

Weiss, K., Gerstenberger, J., Salzig, D., Mühlebach, M. D., Cichutek, K., Pörtner, R. & Czermak, P. (2015). Engineering in Life Sciences, 15(4), 425-436.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs