L-Glutamic Acid Assay Kit

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

Product Code
Content/size
Stock
Price
Qty
K-GLUT
60 assays (manual) / 600 assays (microplate)
/ 700 assays (auto-analyser)
$151.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

Principle:
                         (beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD+ + H2O ↔ 2-oxoglutarate + NADH + NH4+

                         (diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:                             60 assays (manual) / 600 (microplate)
                                          / 700 (auto-analyser)
Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 8 min
Detection limit:                 0.21 mg/L
Application examples:
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by ISO, GOST
and NMKL

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 2 years after preparation
     
  • Glutamate dehydrogenase solution stable at -20°C
     
  • No wasted diaphorase solution (stable suspension supplied)
     
  • Rapid reaction
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Suitable for manual, microplate and auto-analyser formats

Surfactant to dye binding degree based approach for the selective determination of L-glutamate in foodstuffs.

Pedraza, A., Sicilia, M. D., Rubio, S. & Pérez-Bendito, D. (2007). Analytical and Bioanalytical Chemistry, 389(7-8), 2297-2302.

The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism.

El-kereamy, A., Bi, Y. M., Ranathunge, K., Beatty, P. H., Good, A. G. & Rothstein, S. J. (2012). PloS one, 7(12), e52030.

Astrocytic glutamate transporter-dependent neuroprotection against glutamate toxicity: An in vitro study of maslinic acid.

Qian, Y., Guan, T., Tang, X., Huang, L., Huang, M., Li, Y., Sun, H., Yu, R. & Zhang, F. (2011). European Journal of Pharmacology, 651(1-3), 59-65.

Production of stable quinine nanodispersions using esterified γ-polyglutamic acid biopolymer.

Hoennscheidt, C., Kreyenschulte, D., Margaritis, A. & Krull, R. (2013). Biochemical Engineering Journal, 79, 259-266.

Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phylogenetically divergent plants.

Seher, Y., Filiz, O. & Melike, B. (2013). Plant Systematics and Evolution, 299(2), 403-412.

Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate.

Vuoristo, K. S., Mars, A. E., Sangra, J. V., Springer, J., Eggink, G., Sanders, J. P. & Weusthuis, R. A. (2015). AMB Express, 5(1), 61.

Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification.

Stefanovic, E., Kilcawley, K. N., Rea, M. C., Fitzgerald, G. F. & McAuliffe, O. (2017). Journal of Applied Microbiology, 122(5), 1245-1261.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs