L-Glutamic Acid Assay Kit

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

Product Code
60 assays (manual) / 600 assays (microplate)
/ 700 assays (auto-analyser)

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Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

                         (beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD+ + H2O ↔ 2-oxoglutarate + NADH + NH4+

(2) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:    60 assays (manual) / 600 (microplate)
                                          / 700 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 8 min
Detection limit:                 0.21 mg/L
Application examples:
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by ISO, GOST
and NMKL


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Glutamate dehydrogenase solution stable below -10°C
  • No wasted diaphorase solution (stable suspension supplied)
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Surfactant to dye binding degree based approach for the selective determination of L-glutamate in foodstuffs.

Pedraza, A., Sicilia, M. D., Rubio, S. & Pérez-Bendito, D. (2007). Analytical and Bioanalytical Chemistry, 389(7-8), 2297-2302.

The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism.

El-kereamy, A., Bi, Y. M., Ranathunge, K., Beatty, P. H., Good, A. G. & Rothstein, S. J. (2012). PloS one, 7(12), e52030.

Astrocytic glutamate transporter-dependent neuroprotection against glutamate toxicity: An in vitro study of maslinic acid.

Qian, Y., Guan, T., Tang, X., Huang, L., Huang, M., Li, Y., Sun, H., Yu, R. & Zhang, F. (2011). European Journal of Pharmacology, 651(1-3), 59-65.

Production of stable quinine nanodispersions using esterified γ-polyglutamic acid biopolymer.

Hoennscheidt, C., Kreyenschulte, D., Margaritis, A. & Krull, R. (2013). Biochemical Engineering Journal, 79, 259-266.

Gamma-amino butyric acid, glutamate dehydrogenase and glutamate decarboxylase levels in phylogenetically divergent plants.

Seher, Y., Filiz, O. & Melike, B. (2013). Plant Systematics and Evolution, 299(2), 403-412.

Metabolic engineering of the mixed-acid fermentation pathway of Escherichia coli for anaerobic production of glutamate and itaconate.

Vuoristo, K. S., Mars, A. E., Sangra, J. V., Springer, J., Eggink, G., Sanders, J. P. & Weusthuis, R. A. (2015). AMB Express, 5(1), 61.

Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification.

Stefanovic, E., Kilcawley, K. N., Rea, M. C., Fitzgerald, G. F. & McAuliffe, O. (2017). Journal of Applied Microbiology, 122(5), 1245-1261.

Development of a production chain from vegetable biowaste to platform chemicals.

Schmidt, A., Sturm, G., Lapp, C. J., Siebert, D., Saravia, F., Horn, H., Ravi, P. P. Lemmer, A. & Gescher, J. (2018). Microbial Cell Factories, 17(1), 90.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.