L-Glutamine/Ammonia Assay Kit (Rapid)

The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Product Code
50 assays of each (manual) / 500 assays of each (microplate)

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UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials

(1) L-Glutamine + H2O → L-glutamate + NH4+

                         (microbial glutamate dehydrogenase)
(2) NH4+ + 2-Oxoglutarate + NADPH → L-glutamate + NADP+ + H2O

Kit size:    50 assays of each (manual) / 500 assays of each (microplate)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min
Detection limit:                 0.54 mg/L (L-glutamine)
                                         0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition:   Novel method


  • Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
  • All enzymes supplied as stabilised suspensions
  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis.

Barth, C., Gouzd, Z. A., Steele, H. P. & Imperio, R. M. (2010).  Journal of Experimental Botany, 61(2), 379-394.

Metabolic profiling of hematopoietic stem and progenitor cells during proliferation and differentiation into red blood cells.

Daud, H., Browne, S., Al-Majmaie, R., Murphy, W. & Al-Rubeai, M. (2016). New Biotechnology, 33(1), 179-186.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.