L-Malate dehydrogenase (E. coli)
High purity recombinant L-Malate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 1.1.1.37
CAS: 9001-64-3
malate dehydrogenase; (S)-malate:NAD+ oxidoreductase
Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.
Supplied at ~ 15,000 U/mL.
Specific activity: ~ 1,700 U/mg (25oC, pH 7.5. on oxaloacetic acid).
Stability: > 4 years at 4oC.
DESCRIPTION
L-Malate dehydrogenase (Escherichia coli)
EC 1.1.1.37
CAS: 9001-64-3
Synonyms:
malate dehydrogenase; (S)-malate:NAD+ oxidoreductase
Form:
In 3.2 M ammonium sulphate.
Stability:
> 4 years at 4oC.
Specific activity:
~ 1,700 U/mg (25oC, pH 7.5 on oxaloacetic acid).
Unit definition:
One Unit of L-malate dehydrogenase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH in the presence of oxaloacetic acid (0.47 mM) in sodium phosphate buffer (92 mM), pH 7.5 at 25oC.
Specificity:
Catalyses the reaction:
L-Malate + NAD+ = oxaloacetate + NADH + H+
Applications:
Applications for the measurement of L-malic acid in the food, fermentation, wine, beverage and dairy industries.
