Lactulose Assay Kit

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

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Product Code
50 assays per kit

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UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

(1) Lactulose + H2O → D-galactose + D-fructose

            (glucose oxidase + catalase + H2O2)
(2) D-Glucose + H2O + O2 → D-gluconic acid + H2O2

(3) D-Fructose + ATP → F-6-P + ADP

    (phosphoglucose isomerase)
(4) F-6-P           →            G-6-P

       (glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

                    (gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate + NADPH
                                                                                           + CO2 + H+

Kit size:                             50 assays
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 120 min
Detection limit:                 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition:        Novel method


  • Twice the sensitivity of traditional hexokinase based lactulose methods
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

In vitro and in vivo protective effects of fermented preparations of dietary herbs against lipopolysaccharide insult.

Bose, S., Song, M. Y., Nam, J. K., Lee, M. J. & Kim, H. (2012). Food Chemistry, 134(2), 758-765.

The effect of intestinal permeability and endotoxemia on the prognosis of acute pancreatitis.

Koh, Y. Y., Jeon, W. K., Cho, Y. K., Kim, H. J., Chung, W. G., Chon, C. U., Oh, T. Y. & Shin, J. H. (2012). Gut and Liver, 6(4), 505-511.

Assessment of gastrointestinal permeability by lactulose test in sheep after repeated indomethacin treatment.

Minuti, A., Ahmed, S., Trevisi, E., Piccioli-Cappelli, F., Bertoni, G. & Bani, P. (2013). Journal of Animal Science, 91(12), 5646-5653.

Evaluation of the in vitro and in vivo protective effects of unfermented and fermented Rhizoma coptidis formulations against lipopolysaccharide insult.

Bose, S., Jeon, S., Eom, T., Song, M. Y. & Kim, H. (2012). Food Chemistry, 135(2), 452-459.

The effects of co-administration of probiotics with herbal medicine on obesity, metabolic endotoxemia and dysbiosis: A randomized double-blind controlled clinical trial.

Lee, S. J., Bose, S., Seo, J. G., Chung, W. S., Lim, C. Y. & Kim, H. (2013). Clinical Nutrition, 33(6), 973-981.

Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota.

Wang, J. H., Bose, S., Kim, G. C., Hong, S. U., Kim, J. H., Kim, J. E. & Kim, H. (2014). PloS one, 9(1), e86117.

Hepatoprotective effect of Lentinus edodes mycelia fermented formulation against alcoholic liver injury in rats.

Chung, W. S., Wang, J. H., Bose, S., Park, J. M., Park, S. O., Lee, S. J., Jeon, S. & Kim, H. (2015). Journal of Food Biochemistry, 39(3), 251-262.

Evaluation of furosine, lactulose and acid-soluble β-lactoglobulin as time temperature integrators for whipping cream samples at retail in Austria.

Boitz, L. I. & Mayer, H. K. (2015). International Dairy Journal, 50, 24-31.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction & Kit Description
Chapter 2: Process
Chapter 3: Principle
Chapter 4: Reagent Preparation
Chapter 5: Sample Preparation
Chapter 6: Enzymatic Determination Reaction
Chapter 7: Calculations
Chapter 8: ISO Method 11285:2004

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.