Lichenase (endo-1,3:1,4-β-D-Glucanase) (Bacillus subtilis)

High purity Lichenase (endo-1,3,1,4-β-Glucanase) (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH16
CAS: 37288-51-0 

licheninase; (1->3)-(1->4)-beta-D-glucan 4-glucanohydrolase

Highly purified. From Bacillus subtilis.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity:
~ 230 U/mg (40oC, pH 6.5 on barley β-glucan).

Stability: > 4 years at 4oC.

Product Code
5,000 Units

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Lichenase (endo-1,3:1,4-β-D-Glucanase) (Bacillus subtilis)

CAZy Family: GH16

CAS: 37288-51-0

licheninase; (1->3)-(1->4)-beta-D-glucan 4-glucanohydrolase

In 3.2 M ammonium sulphate.

> 4 years at 4oC.

Specific activity:
~ 230 U/mg (40oC, pH 6.5 on barley β-glucan).

Unit definition:
One Unit of lichenase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100mM), pH 6.5 at 40oC.

Hydrolysis of (1,4)-β-D-glucosidic linkages in β-D-glucans containing (1,3)- and (1,4)-bonds.

Applications in carbohydrate research and in the food and feeds, brewing and biofuels industries.

Novel approaches to the automated assay of β-glucanase and lichenase activity.

Mangan, D., Liadova, A., Ivory, R. & McCleary, B. V. (2016). Carbohydrate Research, 435, 162-172.

Measurement of (1→3)(1→4)-β-D-glucan in malt, wort and beer.

McCleary, B. V. & Nurthen, E. (1986). Journal of the Institute of Brewing, 92(2), 168-173.

Enzymic quantification of (1→3) (1→4)-β-D-glucan in barley and malt.

McCleary, B. V. & Glennie-Holmes, M. (1985). Journal of the Institute of Brewing, 91(5), 285-295.

Measurement of (1→3),(1→4)-β-D-glucan in barley and oats: A streamlined enzymic procedure.

McCleary, B. V. & Codd, R. (1991). Journal of the Science of Food and Agriculture, 55(2), 303-312.

Dietary fibers from mushroom sclerotia: 3. In vitro fermentability using human fecal microflora.

Wong, K. H., Wong, K. Y., Kwan, H. S. & Cheung, P. C. K. (2005). Journal of Agricultural and Food Chemistry, 53(24), 9407-9412.

Improved quantitative analysis of oligosaccharides from lichenase-hydrolyzed water-soluble barley β-glucans by high-performance anion-exchange chromatography.

Yoo, D. H., Lee, B. H., Chang, P. S., Lee, H. G. & Yoo, S. H. (2007). Journal of Agricultural and Food Chemistry, 55(5), 1656-1662.

Enzymatic extraction of beta-glucan from oat bran cereals and oat crackers and optimization of viscosity measurement.

Gamel, T. H., Abdel-Aal, E. S. M., Ames, N. P., Duss, R., & Tosh, S. M. (2014). Journal of Cereal Science, 59(1), 33-40.

Textural and Bile Acid-Binding Properties of Muffins Impacted by Oat β-Glucan with Different Molecular Weights.

Sayar, S., Jannink, J. L. & White, P. J. (2011). Cereal Chemistry, 88(6), 564-569.

Identification of quantitative trait loci affecting hemicellulose characteristics based on cell wall composition in a wild and cultivated rice species.

Zhang, S. J., Song, X. Q., Yu, B. S., Zhang, B. C., Sun, C. Q., Knox, J. P. & Zhou, Y. H. (2012). Molecular Plant, 5(1), 162-175.

Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans.

Rieder, A., Grimmer, S., Aachmann, F. L., Westereng, B., Kolset, S. O. & Knutsen, S. H. (2013). Carbohydrate Polymers, 92(2), 2075-2083.

Mechanical and barrier properties of avenin, kafirin, and zein films.

Gillgren, T. & Stading, M. (2008). Food Biophysics, 3(3), 287-294.

Regulation of the cellulose synthase-like gene family by light in the maize mesocotyl.

Van Erp, H. & Walton, J. D. (2009). Planta, 229(4), 885-897.

Effects of barley and oat β-glucan structures on their rheological and thermal characteristics.

Ryu, J. H., Lee, S., You, S., Shim, J. H. & Yoo, S. H. (2012). Carbohydrate Polymers, 89(4), 1238-1243.

In vitro bile-acid binding and fermentation of high, medium, and low molecular weight β-glucan.

Kim, H. J. & White, P. J. (2010). Journal of Agricultural and Food Chemistry, 58(1), 628-634.

How cell wall complexity influences saccharification efficiency in Miscanthus sinensis.

De Souza, A. P., Kamei, C. L. A., Torres, A. F., Pattathil, S., Hahn, M. G., Trindade, L. M. & Buckeridge, M. S. (2015). Journal of Experimental Botany, 66(14), 4351-4365.

Starch structure in developing barley endosperm.

Källman, A., Bertoft, E., Koch, K., Sun, C., Åman, P. & Andersson, R. (2015). International Journal of Biological Macromolecules, 81, 730-735.

Mixed‐Linkage Glucan Oligosaccharides Produced by Automated Glycan Assembly Serve as Tools to Determine the Substrate Specificity of Lichenase.

Dallabernardina, P., Schuhmacher, F., Seeberger, P. H. & Pfrengle, F. (2017). Chemistry-A European Journal, 23(13), 3191-3196.

Improvement of enzyme activity of β-1,3-1,4-glucanase from Paenibacillus sp. X4 by error-prone PCR and structural insights of mutated residues.

Baek, S. C., Ho, T. H., Lee, H. W., Jung, W. K., Gang, H. S., Kang, L. W. & Kim, H. (2017). Applied Microbiology and Biotechnology, 101(10), 4073-4083.

Impact of hydrothermal and mechanical processing on dissolution kinetics and rheology of oat β-glucan.

Myriam, M. L. G., Quint, J., Rieder, A., Ballance, S., Dreiss, C. A., Butterworth, P. J. & Ellis, P. R. (2017). Carbohydrate Polymers, 166, 387-397.

The impact of oat structure and &beta-glucan on in vitro lipid digestion.

Grundy, M. M. L., Quint, J., Rieder, A., Ballance, S., Dreiss, C. A., Butterworth, P. J. & Ellis, P. R. (2017). Carbohydrate Polymers, 166, 387-397.

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