Limit-Dextrizyme Tablets

High purity dyed and crosslinked Limit-Dextrizyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

For the assay of limit-dextrinase and pullulanase. Containing AZCL-Pullulan.

New updated assay protocol employing amyloglucosidase into sample preparation.

Limit-Dextrizyme Tablets
Product Code
200 Tablets
1,000 Tablets

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Validation of Methods

RACI Standard Method

Colourimetric and fluorimetric substrates for the assay of limit dextrinase.

Mangan, D., McCleary, B. V., Cornaggia, C., Ivory, R., Rooney, E. & McKie, V. (2015). Journal of Cereal Science, 62, 50-57.

Measurement of the content of limit-dextrinase in cereal flours.

McCleary, B. V. (1992). Carbohydrate Research, 227, 257-268.

Detection of a limit dextrinase inhibitor in barley.

Macri, L. J., MacGregor, A. W., Schroeder, S. W. & Bazin, S. L. (1993). Journal of Cereal Science, 18(2), 103-106.

Purification and characterisation of limit dextrinase inhibitors from barley.

MacGregor, A. W., Macri, L. J., Schroeder, S. W. & Bazin, S. L. (1994). Journal of Cereal Science, 20(1), 33-41.

Limit dextrinase from germinating barley has endotransglycosylase activity, which explains its activation by maltodextrins.

McDougall, G. J., Ross, H. A., Swanston, J. S. & Davies, H. V. (2004). Planta, 218(4), 542-551.

Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris.

Jensen, J. M., Vester-Christensen, M. B., Møller, M. S., Bønsager, B. C., Christensen, H. E. M., Hachem, M. A. & Svensson, B. (2011). Protein Expression and Purification, 79(2), 217-222.

The survival of limit dextrinase during fermentation in the production of Scotch whisky.

Walker, J. W., Bringhurst, T. A., Broadhead, A. L., Brosnan, J. M. & Pearson, S. Y. (2001). Journal of the Institute of Brewing, 107(2), 99-106.

Thioredoxin in barley: could it have a role in releasing limit dextrinase in brewery mashes?

Heisner, C. B. & Bamforth, C. W. (2008). Journal of the Institute of Brewing, 114(2), 122-126.

Secretory expression of functional barley limit dextrinase by Pichia pastoris using high cell-density fermentation.

Vester-Christensen, M. B., Hachem, M. A., Naested, H. & Svensson, B. (2010). Protein Expression and Purification, 69(1), 112-119.

The advantages of using natural substrate‐based methods in assessing the roles and synergistic and competitive interactions of barley malt starch‐degrading enzymes.

Osman, A. M. (2002). Journal of the Institute of Brewing, 108(2), 204-214.

Oligosaccharide and substrate binding in the starch debranching enzyme barley limit dextrinase.

Møller, M. S., Windahl, M. S., Sim, L., Bøjstrup, M., Hachem, M. A., Hindsgaul, O., Palcic, M., Svensson, B. & Henriksen, A. (2015). Journal of Molecular Biology, 427(6), 1263-1277.

A chromogenic assay suitable for high-throughput determination of limit dextrinase activity in barley malt extracts.

Bøjstrup, M., Marri, L., Lok, F. & Hindsgaul, O. (2015). Journal of agricultural and Food Chemistry, 63(50), 10873-10878.

Optimization of the production of an extracellular and thermostable amylolytic enzyme by Thermus thermophilus HB8 and basic characterization.

Akassou, M. & Groleau, D. (2017). Extremophiles, 1-14.
Analysis of enzymes activity using carbohydrase tablet testing

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Theory of endo-1, 4-Beta-D-Xylanase Assay Procedure
Chapter 2: Buffers & Reagents
Chapter 3: Assay Procedure

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