Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

Malt β-glucanase:                  
100 assays (manual format) / 400 assays (auto-analyser format)
Lichenase:                           
100 / 200 assays (manual format) / 330 assays (auto-analyser format)

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage
β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Data calculators are located in the Documentation tab.

Product Code
Content/size
Stock
Price
Qty
K-MBG4
100 assays (manual) / 400 assays (auto-analyser)
$295.00

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Colourimetric method for the determination of endo-1,3:1.4-β-glucanase/lichenase in crude malt extracts and enzyme preparations

Principle:
                                       (endo-1,3:1,4-β-glucanase/lichenase)
(1) Benzylidene-BGTETB-β-CNP + H2O → Benzymidene-BGTETB + CNP

         (tris buffer)
(2) CNP → phenolate ion (yellow colour)
Note: CNP = 2-Chloro-4-nitrophenol

Kit size:
Malt β-glucanase 100 assays (manual) / 400 (auto-analyser)
                                        or
Lichenase 100 / 200 assays (manual) / 330 (auto-analyser)

Method:                         Spectrophotometric at 400 nm
Total assay time:           
Malt β-glucanase 20 min (manual) / 10 min (auto-analyser)
                                        or
Lichenase 10 min (manual) / 10 min (auto-analyser) 
Detection limit:
Malt β-glucanase 4.3 x 10-4 U/mL
                                         or
Lichenase 9.1 x 10-5 U/mL
Application examples:
Crude malt extracts, industrial enzyme preparations 
Method recognition:     Novel method

Advantages

  • Very cost effective
     
  • All reagents stable for > 2 years
     
  • Specific for endo-1,3:1,4-β-glucanase/lichenase
     
  • Simple, convenient, rapid assay
     
  • Well suited to automation
     
  • Malt flour standard and lichenase standard included

Novel approaches to the automated assay of β-glucanase and lichenase activity.

Mangan, D., Liadova, A., Ivory, R. & McCleary, B. V. (2016). Carbohydrate Research, 435, 162-172.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs