Mannitol dehydrogenase (Pseudomona fluorescens

High purity recombinant Mannitol dehydrogenase (Pseudomona fluorescens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 1.1.1.67
CAS: 9001-65-4

mannitol 2-dehydrogenase; D-mannitol:NAD+ 2-oxidoreductase

Recombinant. From Pseudomona fluorescens.
In 3.2 M ammonium sulphate.
Supplied at ~ 300 U/mL. 

Specific activity:
~ 50 U/mg (pH 8.6, 40oC on D-mannitol).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-MNHPF
500 Units
$177.00

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DESCRIPTION

Mannitol dehydrogenase (Pseudomona fluorescens)

EC 1.1.1.67
CAS: 9001-65-4

Synonyms:
mannitol 2-dehydrogenase; D-mannitol:NAD+ 2-oxidoreductase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 50 U/mg (pH 8.6, 40oC on D-mannitol).

Unit definition:
One Unit of mannitol dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ in the presence of D-mannitol (5.2 mM) in Tris-HCl buffer (64 mM), pH 8.6 at 25oC.

Specificity:
Catalyses the reaction:
D-mannitol + NAD+ = D-fructose + NADH + H+

Applications:
Low Km, suitable for D-mannitol determination.
Potential uses in the biotechnology industry.