Pectate Lyase (Aspergillus sp.) 

High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 4.2.2.2
CAZy Family: PL1
CAS: 9015-75-2 

pectate lyase; (1->4)-alpha-D-galacturonan lyase

From Aspergillus sp.
In 3.2 M ammonium sulphate.
Supplied at ~ 3,000 U/mL. 

Specific activity:
~ 180 U/mg (40oC, pH 8.0 on polygalacturonic acid).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-PCLYAN2
7,500 Units in 3.2 M ammonium sulphate
$182.00

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DESCRIPTION

Pectate lyase (Aspergillus sp.)

EC 4.2.2.2
CAZy Family: PL1

CAS: 9015-75-2

Synonyms:
pectate lyase; (1->4)-alpha-D-galacturonan lyase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 180 U/mg (40oC, pH 8.0 on polygalacturonic acid).

Unit definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40oC.

Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

Applications:
Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.

Complete Chemical Analysis of Carmagnola Hemp Hurds and Structural Features of Its Components.

Gandolfi, S., Ottolina, G., Riva, S., Fantoni, G. P. & Patel, I. (2013). BioResources, 8(2), 2641-2656.