Peroxidase (NADH peroxidase) (Enterococcus faecalis)
High purity recombinant NADH peroxidase (Enterococcus faecalis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 1.11.1.1
CAS: 9032-24-0
NADH peroxidise; NADH:hydrogen-peroxide oxidoreductase
Recombinant. From Enterococcus faecalis.
In 50% (v/v) glycerol.
Supplied at ~ 400 U/mL.
Specific activity:
~ 30 U/mg (25oC, pH 5.5 on NADH in the presence of hydrogen peroxide).
Stability: > 2 years at -20oC.

DESCRIPTION
Peroxidase (NADH peroxidase) (Enterococcus faecalis)
EC 1.11.1.1
CAS: 9032-24-0
Synonyms:
NADH peroxidise; NADH:hydrogen-peroxide oxidoreductase
Form:
In 50% (v/v) glycerol.
Stability:
> 2 years at -20oC.
Specific activity:
~ 30 U/mg (25oC, pH 5.5 on NADH in the presence of hydrogen peroxide).
Unit definition:
One Unit of NADH peroxidase activity is defined as the amount of enzyme required to oxidise one µmole of NADH to NAD+ per minute in the presence of hydrogen peroxide (2 mM) in sodium acetate buffer (100 mM), pH 5.5 at 25oC.
Specificity:
Catalyses the reaction:
NADH + H+ + H2O2 = NAD+ + 2H2O
Applications:
Used widely in diagnostic and analytical applications.