Phosphotransacetylase (Bacillus subtilis)

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAS: 9029-91-8 

phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

Recombinant. From Bacillus subtilis.
In 3.2 M ammonium sulphate.
Supplied at ~ 3,000 U/mL. 

Specific activity: 
~ 2,000 U/mg (25oC, pH 7.4 on Coenzyme A).

Stability: Minimum 1 year at 4oC. Check vial for details.

Product Code
3,000 Units

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Phosphotransacetylase (Bacillus subtilis)

CAS: 9029-91-8

phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

In 3.2 M ammonium sulphate.

Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 2,000 U/mg (25oC, pH 7.4 on coenzyme A).

Unit definition:
One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25oC.

Catalyses the reaction:
Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.