Protease Subtilisin A (from Bacillus licheniformis) Powder 

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.4.21.62
CAS: 9014-01-1

subtilisin; subtilisin A

Highly purified. From Bacillus licheniformis.
In p
owder form. For use in Megazyme Total Dietary Fiber test method. 

Specific activity:
> 10 U/mg of protein; (40oC, pH 8.0 on casein). 

Stability: > 4 years at -20oC.

View Megazyme’s latest Guide for Dietary Fiber Analysis.

Product Code
Content/size
Stock
Price
Qty
E-BSPRPD
1 gram, powder
$216.00

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DESCRIPTION

Protease (Subtilisin A from Bacillus licheniformis) Powder 

EC 3.4.21.62
CAS: 9014-01-1

Synonyms:
subtilisin; subtilisin A  

Form:
In powder form.

Stability: 
> 4 years at -20oC.

Specific activity:
> 10 U/mg of protein; (40oC, pH 8.0 on casein). 

Unit definition:
One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). 

Specificity:
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides.

Applications:
This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fibre analytical procedures.

Investigation of folic acid stability in fortified instant Asian noodles by use of capillary electrophoresis.

Cheung, R. H. F., Hughes, J. G., Marriott, P. J. & Small, D. M. (2009). Food Chemistry, 112(2), 507-514.

Determination of folate contents in some Australian vegetables.

Iwatani, Y., Arcot, J. & Shrestha, A. K. (2003). Journal of Food Composition and Analysis, 16(1), 37-48.

Folates in Asian noodles: II. A comparison of commercial samples and the impact of cooking.

Bui, L. T. T. & Small, D. M. (2007). Journal of Food Science, 72(5), C283-C287.