Proteinase K (Molecular Biology Grade)

High purity Proteinase K (Molecular Biology Grade) from Parengyodontium album (Tritirachium album) for use in research and analytical applications.

EC 3.4.21.62
CAS: 39450-01-6

Subtilisin

Recombinant. From Parengyodontium album (Tritirachium album).
Electrophoretically homogeneous, pI 8.3.
In powder form.

Specific activity: 
> 40 U/mg protein (37oC, pH 7.5 on urea-denatured haemoglobin).

Stability: > 2 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-PRKMB
500 mg
$325.00

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DESCRIPTION

Proteinase K (Molecular Biology Grade)

EC 3.4.21.62
CAS: 39450-01-6

Synonyms:
Subtilisin

Form:
In powder form.

Stability: 
> 2 years at -20oC.

Specific activity:
> 40 U/mg protein (37oC, pH 7.5 on urea-denatured haemoglobin).

Unit definition:
One Unit of Proteinase K will hydrolyse urea-denatured hemoglobin to produce a colour (by Folin-Ciocalteu reagent) equivalent to 1.0 μmole of L-tyrosine per min at pH 7.5 and 37oC.  1 U = 1 mAnsonU.

Specificity:
Proteinase K from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease with broad substrate specificity for hydrolysis of peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. It is able to hydrolyse peptide bonds in the presence of Triton, SDS, urea and EDTA.

Applications:
Proteinase K can be used for removal or inactivation of proteins in various analytical or research applications. It is used to inactivate nucleases during isolation of DNA and RNA. Activity in the presence of EDTA permits the use of EDTA in the purification of DNA or RNA to inhibit calcium dependent nucleases.