Raffinose/D-Galactose Assay Kit

The Raffinose/D-Galactose test kit is specific and a rapid measurement and analysis of raffinose and D-galactose in plant materials and food products.

Product Code
120 assays per kit

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UV-method for the determination of Raffinose (also stachyose
and verbascose) and D-Galactose in legume seeds, plant
materials, foodstuffs and feed

(1) Raffinose + stachyose + verbascose + H2O → D-galactose +

               (galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose

                     (β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                            120 assays
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 60 min
Detection limit:                 21 mg/L
Application examples:
Cereal flours, soybean flour, by-products of sucrose manufacture
and other materials
Method recognition:    
Used and accepted in food analysis


  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum).

Zha, H. G., Flowers, V. L., Yang, M., Chen, L. Y. & Sun, H. (2012). Annals of botany, 109(4), 735-745.

Viscozyme L action on soy slurry affects carbohydrates and antioxidant properties of silken tofu.

Rosset, M., Prudencio, S. H. & Beléia, A. D. P. (2012). Food Science and Technology International, 18(6), 531-538.

Effects of a brown beans evening meal on metabolic risk markers and appetite regulating hormones at a subsequent standardized breakfast: a randomized cross-over study.

Nilsson, A., Johansson, E., Ekström, L. & Björck, I. (2013). PloS one, 8(4), e59985.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.