Raffinose/D-Galactose Assay Kit

The Raffinose/D-Galactose test kit is specific and a rapid measurement and analysis of raffinose and D-galactose in plant materials and food products.

Product Code
120 assays per kit

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UV-method for the determination of Raffinose (also stachyose
and verbascose) and D-Galactose in legume seeds, plant
materials, foodstuffs and feed

(1) Raffinose + stachyose + verbascose + H2O → D-galactose +

               (galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose

                     (β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                            * 120 assays

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 60 min
Detection limit:                 21 mg/L
Application examples:
Cereal flours, soybean flour, by-products of sucrose manufacture
and other materials
Method recognition:    
Used and accepted in food analysis


  • Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Acidic α-galactosidase is the most abundant nectarin in floral nectar of common tobacco (Nicotiana tabacum).

Zha, H. G., Flowers, V. L., Yang, M., Chen, L. Y. & Sun, H. (2012). Annals of botany, 109(4), 735-745.

Viscozyme L action on soy slurry affects carbohydrates and antioxidant properties of silken tofu.

Rosset, M., Prudencio, S. H. & Beléia, A. D. P. (2012). Food Science and Technology International, 18(6), 531-538.

Effects of a brown beans evening meal on metabolic risk markers and appetite regulating hormones at a subsequent standardized breakfast: a randomized cross-over study.

Nilsson, A., Johansson, E., Ekström, L. & Björck, I. (2013). PloS one, 8(4), e59985.

The effects of fermentation and enzymatic treatment of pea on nutrient digestibility and growth performance of broilers.

Boroojeni, F. G., Senz, M., Kozłowski, K., Boros, D., Wisniewska, M., Rose, D., Männer, K. & Zentek, J. (2017). Animal, 1-10.

Arabidopsis galactinol synthases 1 (AtGOLS1) negatively regulates seed germination.

Jang, J. H., Shang, Y., Kang, H. K., Kim, S. Y., Kim, B. H. & Nam, K. H. (2018). Plant Science, 267, 94-101.

Fermentation and enzymatic treatment of pea for turkey nutrition.

Boroojeni, F. G., Kozłowski, K., Jankowski, J., Senz, M., Wiśniewska, M., Boros, D., Drażbo, A. & Zentek, J. (2018). Animal Feed Science and Technology, In Press.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.