Raffinose/Sucrose/D-Glucose Assay Kit

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Product Code
120 assays of each per kit

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Colourimetric method for the determination of Raffinose (also
stachyose and verbascose), Sucrose and D-Glucose in
legume seeds, plant materials, foodstuffs and feed

(1) Raffinose + stachyose + verbascose + H2O → D-galactose +

(2) Sucrose + H2O → D-glucose + D-fructose

                                 (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                            quinoneimine + 4H2O

Kit size:                            120 assays of each per kit
Method:                           Spectrophotometric at 510 nm
Reaction time:                 ~ 20 min
Detection limit:                100 mg/L
Application examples:
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition:         
Used and accepted in food analysis


  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

High hydrostatic pressure influences antinutritional factors and in vitro protein digestibility of split peas and whole white beans.

Linsberger-Martin, G., Weiglhofer, K., Thi Phuong, T. P. & Berghofer, E. (2013). LWT-Food Science and Technology, 51(1), 331-336.

Differences in chemical composition of field pea (Pisum sativum) cultivars: Effects of cultivation area and year.

Nikolopoulou, D., Grigorakis, K., Stasini, M., Alexis, M. N. & Iliadis, K. (2007). Food Chemistry, 103(3), 847-852.

Growth, feed utilization, health and organoleptic characteristics of European seabass (Dicentrarchus labrax) fed extruded diets including low and high levels of three different legumes.

Adamidou, S., Nengas, I., Henry, M., Grigorakis, K., Rigos, G., Nikolopoulou, D., Kotzamanis, Y., Bell, G. J. & Jauncey, K. (2009). Aquaculture, 293(3-4), 263-271.

Effects of cultivation area and year on proximate composition and antinutrients in three different kabuli-type chickpea (Cicer arientinum) varieties.

Nikolopoulou, D., Grigorakis, K., Stasini, M., Alexis, M. & Iliadis, K. (2006). European Food Research and Technology, 223(6), 737-741.

Moisture deficit stress affects yield and quality in groundnut seeds.

Chakraborty, K., Bishi, S. K., Singh, A. L., Kalariya, K. A. & Kumar, L. (2013). Indian Journal of Plant Physiology, 18(2), 136-141.

Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation.

Patton, A. J., Cunningham, S. M., Volenec, J. J. & Reicher, Z. J. (2007). Crop Science, 47(5), 2170-2181.

Characterization of Spanish peanut germplasm (Arachis hypogaea L.) for sugar profiling and oil quality.

Bishi, S. K., Kumar, L., Dagla, M. C., Mahatma, M. K., Rathnakumar, A. L., Lalwani, H. B. & Misra, J. B. (2013). Industrial Crops and Products, 51, 46-50.

Freezing tolerance attributes during spring deacclimation for three asparagus cultivars with varying adaptation to southern Ontario.

Panjtandoust, M. & Wolyn, D. J. (2016). Journal of the American Society for Horticultural Science, 141(1), 22-33.

Galacto-oligosaccharide Hydrolysis by Genetically-Engineered Alpha-Galactosidase-Producing Pseudomonas chlororaphis Strains.

Solaiman, D. K. Y., Ashby, R. D., Aneja, K. K., Crocker, N. V. & Liu, Y. (2017). Biocatalysis and Agricultural Biotechnology, 13, 213-218.

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