Resistant Starch Assay Kit

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.

Product Code
100 assays per kit

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Validation of Methods

AACC Method 32-40.01


AOAC Method 2002.02


CODEX Method Type II

Colourimetric method for the determination of Resistant Starch
in cereal products and feeds

                              (α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose +

(3) Dissolution of resistant starch pellet in KOH and neutralisation

                                         (α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H2O → D-glucose

                               (glucose oxidase)
(5) D-Glucose + H2O + O2 → D-gluconate + H2O2

(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                         quinoneimine + 4H2O

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Reaction time:                  ~ 120 min (plus overnight incubation)
Detection limit:                 2-100% of sample weight
Application examples:
Plant materials, starch samples and other materials
Method recognition:    
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)


  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Measures enzyme resistant starch
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Two issues in dietary fiber measurement.

McCleary, B. V. (2001). Cereal Foods World, 46(4), 164-165.

Measurement of resistant starch.

McCleary, B. V. & Monaghan, D. A. (2002). Journal of AOAC International, 85(3), 665-675.

Measurement of resistant starch by enzymatic digestion in starch and selected plant materials: Collaborative study.

McCleary, B. V., McNally, M. & Rossiter, P. (2002). Journal of AOAC International, 85(5), 1103-1111.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

An integrated procedure for the measurement of total dietary fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates.

McCleary, B. V. (2007). Analytical and Bioanalytical Chemistry, 389(1), 291-308.

Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination.

Xia, H., Yandeau-Nelson, M., Thompson, D. B. & Guiltinan, M. J. (2011). BMC Plant Biology, 11(1), 95-107.

Improvement of the AOAC 2009.01 total dietary fibre method for bread and other high starch containing matrices.

Brunt, K. & Sanders, P. (2013). Food Chemistry, 140(3), 574-580.

Sterilization in a liquid of a specific starch makes it slowly digestible in vitro and low glycemic in rats.

Severijnen, C., Abrahamse, E., Van der Beek, E. M., Buco, A., van de Heijning, B. J. M., van Laere, K. & Bouritius, H. (2007). The Journal of Nutrition, 137(10), 2202-2207.

Effects of wheat inclusion and xylanase supplementation of the diet on productive performance, nutrient retention, and endogenous intestinal enzyme activity of laying hens.

Mirzaie, S., Zaghari, M., Aminzadeh, S., Shivazad, M. & Mateos, G. G. (2012). Poultry Science, 91(2), 413-425.

Exotic corn lines with increased resistant starch and impact on starch thermal characteristics.

Rohlfing, K. A., Pollak, L. M. & White, P. J. (2010). Cereal Chemistry, 87(3), 190-193.

Effect of extrusion conditions on resistant starch formation from pastry wheat flour.

Kim, J. H., Tanhehco, E. J. & Ng, P. K. W. (2006). Food Chemistry, 99(4), 718-723.

Resistant starch and starch pasting properties of a starch synthase IIa-deficient wheat with apparent high amylose.

Yamamori, M., Kato, M., Yui, M. & Kawasaki, M. (2006). Australian Journal of Agricultural Research, 57(5), 531-535.

Effects of process variables and addition of polydextrose and whey protein isolate on the properties of barley extrudates.

Kirjoranta, S., Solala, K., Suuronen, J. P., Penttilä, P., Peura, M., Serimaa, R., Tenkanen, M. & Jouppila, K. (2012). International Journal of Food Science & Technology, 47(6), 1165-1175.

Elimination of resistant starch type II within the framework of total starch and dietary fibre analysis by microwave irradiation.

Themeier, H., Hollmann, J., Neese, U. & Lindhauer, M. G. (2010). Quality Assurance and Safety of Crops & Foods, 2(1), 46-51.

Resistant starch in Micronesian banana cultivars offers health benefits.

Thakorlal, J., Perera, C. O., Smith, B., Englberger, L. & Lorens, A. (2010). Pacific Health Dialog, 16(1), 49-59.

Enzyme susceptibility of high-amylose starch precipitated from sodium hydroxide dispersions.

Evans, A. & Thompson, D. B. (2008). Cereal Chemistry, 85(4), 480-487.

In vitro fermentation of spent turmeric powder with a mixed culture of pig faecal bacteria.

Han, K. H., Azuma, S. & Fukushima, M. (2014). Food & Function, 10, 2446-2452.

Impact of ancient cereals, pseudocereals and legumes on starch hydrolysis and antiradical activity of technologically viable blended breads.

Collar, C., Jiménez, T., Conte, P. & Fadda, C. (2014). Carbohydrate Polymers, 113, 149-158.

Characterization and Prebiotic Effect of the Resistant Starch from Purple Sweet Potato.

Zheng, Y., Wang, Q., Li, B., Lin, L., Tundis, R., Loizzo, M. R., Zheng, B. & Xiao, J. (2016). Molecules, 21(7), 932.

Metabolomic and transcriptomic responses induced in the livers of pigs by the long-term intake of resistant starch.

Sun, Y., Yu, K., Zhou, L., Fang, L., Su, Y. & Zhu, W. (2016). Journal of animal science, 94(3), 1083-1094.

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