Succinyl-CoA synthetase (prokaryote) 

High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 6.2.1.5
CAS: 9080-33-5 

succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)

Recombinant. From a prokaryotic source.
In 3.2 M ammonium sulphate.
Supplied at ~ 220 U/mL. 

Specific activity:
~ 8 U/mg (25oC, pH 8.4 on succinic acid).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-SCOAS
550 Units
$221.00

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DESCRIPTION

Succinyl-CoA synthetase (prokaryote)

EC 6.2.1.5
CAS: 9080-33-5

Synonyms:
succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 8 U/mg (25oC, pH 8.4 on succinic acid).

Unit definition:
One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25oC. 

Specificity:
Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.

Applications:
Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.