Sucrase plus β-Galactosidase 

High purity Sucrase (maltase; Bacillus stearothermophilis) / β-Galactosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.20 (Sucrase)
CAZy Family: GH13
CAS: 9001-42-7

alpha-glucosidase; alpha-D-glucoside glucohydrolase

EC 3.2.1.23 (β-Galactosidase)
CAZy Family: GH35
CAS: 9031-11-2

beta-galactosidase; beta-D-galactoside galactohydrolase

Sucrase: From Bacillus stearothermophilis.
β-Galactosidase: From Aspergillus niger.
Freeze-dried powder.

Activity/Concentration:
Sucrase (170 U) / β-Galactosidase (3,000 U)

Stability: > 4 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-SUCRBG
170 U Sucrase plus 3,000 U β-Galactosidase
$187.00

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DESCRIPTION

Sucrase plus β-Galactosidase

EC 3.2.1.20 (Sucrase)
CAZy Family: GH13

CAS: 9001-42-7

EC 3.2.1.23 (β-Galactosidase)
CAZy Family: GH35

CAS: 9031-11-2

Synonyms:
(Sucrase): alpha-glucosidase; alpha-D-glucoside glucohydrolase
(β-Galactosidase): beta-galactosidase; beta-D-galactoside galactohydrolase

Form:
Freeze-dried powder.

Stability: 
> 4 years at -20oC.

Activity/Concentration
Sucrase: 170 U.
β-Galactosidase: 3,000 U.

Unit definition:
One Unit of sucrase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from sucrose (10 mM) in sodium phosphate buffer (100 mM), pH 6.8 at 40oC. 

One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Specificity:
Sucrase/ β-galactosidase mixture:
This enzyme mixture gives complete hydrolysis of sucrose, maltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf.

Applications:
Used for removal of sucrose, maltose and lactose in the measurement of dietary fibre.

Efficacy of reducing sugar and phenol–sulfuric acid assays for analysis of soluble carbohydrates in feedstuffs.

Hall, M. B. (2013). Animal Feed Science and Technology, 185(1), 94-100.