Total Starch Assay Kit (AA/AMG) 

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products.  This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

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Product Code
50 assays
100 assays

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Validation of Methods

AACC Method 76-13.01


AOAC Method 996.11


ICC Standard No. 168


RACI Standard Method

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

                      (α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(2) Maltodextrins + H2O → D-glucose

                                 (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                       quinoneimine + 4H2O

Kit size:                               50/100 assays
Method:                               Spectrophotometric at 510 nm
Total assay time:                ~ 90 min
Detection limit:                   1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:    
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168) and RACI (Standard Method)


  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Quantitative measurement of total starch in cereal flours and products.

McCleary, B. V., Solah, V. & Gibson, T. S. (1994). Journal of Cereal Science, 20(1), 51-58.

Total starch measurement in cereal products: interlaboratory evaluation of a rapid enzymic test procedure.

McCleary, B. V., Gibson, T. S., Solah, V. & Mugford, D. C. (1994). Cereal Chemistry, 71(5), 501-505.

Collaborative evaluation of a simplified assay for total starch in cereal products (AACC Method 76-13).

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Cereal Foods World, 42, 476-480.

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study.

McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food.

McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Measurement of Starch: Critical evaluation of current methodology.

B. V. McCleary, L. M. J. Charmier & V. A. McKie. (2018). Starch‐Stärke, 71(1-2), 1800146.

Sourdough bread: Starch digestibility and postprandial glycemic response.

Scazzina, F., Del Rio, D., Pellegrini, N. & Brighenti, F. (2009). Journal of Cereal Science, 49(3), 419-421.

Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition.

Laurens, L. M., Van Wychen, S., McAllister, J. P., Arrowsmith, S., Dempster, T. A., McGowen, J. & Pienkos, P. T. (2014). Analytical Biochemistry, 452, 86-95.

Production of high-starch duckweed and its conversion to bioethanol.

Xu, J., Cui, W., Cheng, J. J. & Stomp, A. M. (2011). Biosysystems engineering, 110(2), 67-72.

High hydrostatic pressure influences antinutritional factors and in vitro protein digestibility of split peas and whole white beans.

Linsberger-Martin, G., Weiglhofer, K., Thi Phuong, T. P. & Berghofer, E. (2013). LWT-Food Science and Technology, 51(1), 331-336.

Effect of supplementary concentrate type on nitrogen partitioning in early lactation dairy cows offered perennial ryegrass-based pasture.

Whelan, S. J., Pierce, K. M., McCarney, C., Flynn, B. & Mulligan, F. J. (2012). Journal of Dairy Science, 95(8), 4468-4477.

Brewer’s spent grain as a functional ingredient for breadsticks.

Ktenioudaki, A., Chaurin, V., Reis, S. F. & Gallagher, E. (2012). International Journal of Food Science & Technology, 47(8), 1765-1771.

Effect of forage source and a supplementary methionine hydroxy analog on nitrogen balance in lactating dairy cows offered a low crude protein diet.

Whelan, S. J., Mulligan, F. J., Flynn, B., McCarney, C. & Pierce, K. M. (2011). Journal of Dairy Science, 94(10), 5080-5089.

Analysis of ADP-glucose pyrophosphorylase expression during turion formation induced by abscisic acid in Spirodela polyrhiza (greater duckweed).

Wang, W. & Messing, J. (2012). BMC plant biology, 12(1), 5.

Buckwheat starch: structure, functionality and enzyme in vitro susceptibility upon the roasting process.

Christa, K., Soral-Smietana, M. & Lewandowicz, G. (2009). International Journal of Food Sciences and Nutrition, 60(s4), 140-154.

Deficiency of maize starch-branching enzyme i results in altered starch fine structure, decreased digestibility and reduced coleoptile growth during germination.

Xia, H., Yandeau-Nelson, M., Thompson, D. B. & Guiltinan, M. J. (2011). BMC Plant Biology, 11(1), 95-107.

Profiling brewers' spent grain for composition and microbial ecology at the site of production.

Robertson, J. A., I'Anson, K. J. A., Treimo, J., Faulds, C. B., Brocklehurst, T. F., Eijsink, V. G. H. & Waldron, K. W. (2010). LWT-Food Science and Technology, 43(6), 890-896.

Enzymatic solubilization of brewers’ spent grain by combined action of carbohydrases and peptidases.

Treimo, J., Westereng, B., Horn, S. J., Forssell, P., Robertson, J. A., Faulds, C. B., Waldron K. W., Buchert J. & Eijsink, V. G. (2009). Journal of Agricultural and Food Chemistry, 57(8), 3316-3324.

Effect of sorghum flour addition on in vitro starch digestibility, cooking quality, and consumer acceptability of durum wheat pasta.

Khan, I., Yousif, A. M., Johnson, S. K. & Gamlath, S. (2014). Journal of Food Science, 79(8), S1560-S1567.

Physicochemical properties of starch from sago (metroxylon sagu) palm grown in mineral soil at different growth stages.

Uthumporn, U., Wahidah, N. & Karim, A. A. (2014). IOP Conference Series: Materials Science and Engineering (Vol. 62, No. 1, p. 012026). IOP Publishing.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.