Total Sulfite Assay Kit

The Total Sulphite test kit is a rapid, simple, reliable and accurate method for the measurement and analysis of total sulfite (sulphite) in wine, beverages, food stuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual, auto-analyser and microplate formats.

Suitable for manual, auto-analyser and microplate formats.

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Product Code
80 assays (manual) / 800 assays (microplate)
/ 800 assays (auto-analyser)

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Colourimetric methods for the determination of Total Sulfite
in wine, fruit juice, food stuffs and other materials

The Total Sulfite assay is based on the reaction principle between thiol groups and Ellman’s reagent

Kit size:                              80 assays (manual) / 800 (microplate)
                                            / 800 (auto-analyser)
Method:                             Spectrophotometric at 405 nm
Total assay time:              ~ 6 min
Detection limit:                 ~ 5 mg/L
Application examples:
Wine, fruit juice, sea food, food stuffs and other materials
Method recognition:    
Validated for red and white wines at the Bundesamt für Weinbau, Austria.
Used widely in the wine industry


  • ”Ready to use" liquid stable formulation
  • Very competitive price (cost per test)
  • All reagents stable for > 18 months
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction & Kit Description
Chapter 2: Theory of Assay Procedure
Chapter 3: Standards & Calibration Curves
Chapter 4: Preparation of the Single Point Standard
Chapter 5: Preparation of the Calibration Curve
Chapter 6: Assay Procedure
Chapter 7: Total Sulphite Calculations

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.