Total Sulfite Assay Kit (Enzymatic)

The Total Sulfite (Enzymatic) test kit is a simple, highly specific and reliable, enzymatic method for the measurement and analysis of total sulfite (as SO2) in wines, beverages, foodstuffs and other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

Image unavailable
Product Code
Content/size
Stock
Price
Qty
K-ETSULPH
50 assays (manual) / 500 assays (microplate)
/ 588 assays (auto-analyser)
$224.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

UV-method for the determination of Total Sulfite (SO22-) in
beverages, foodstuffs and other materials

Principle:
                      (sulfite oxidase)
(1) SO32- + O2 + H2O ↔ SO42- + H2O2

                         (NADH - peroxidase)
(2) H2O2 + NADH + H+ → 2H2O + NAD+

Kit size:                             * 50 assays (manual) / 500 (microplate)
                                           / 588 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Total assay time:              ~ 30 min
Detection limit:                 0.3 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, fruit and vegetables,
pharmaceuticals and other materials
Method recognition:    
Methods based on this principle have been accepted by DIN, EN,
MEBAK and NMKL

Advantages

  • Very cost effective
     
  • All reagents stable for > 2 years during use
     
  • Simple format
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included
     
  • Extended cofactors stability
     
  • Suitable for manual, microplate and auto-analyser formats
The training video below demonstrates some general principles of wine analysis.

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Introduction
Chapter 2: MegaQuant Assay Format
Chapter 3: Manual Format – Recording Spectrophotometer
Chapter 4: Manual Format –Non Recording Spectrophotometer
Chapter 5: Autoanalyser Format
Chapter 6: Liquid Ready Reagents
Chapter 7: Sample Preparation/PVPP Treatment

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs