Total Sulfite Assay Kit (Enzymatic)

The Total Sulfite (Enzymatic) test kit is a simple, highly specific and reliable, enzymatic method for the measurement and analysis of total sulfite (as SO2) in wines, beverages, foodstuffs and other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

Product Code
50 assays (manual) / 500 assays (microplate)
/ 588 assays (auto-analyser)

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UV-method for the determination of Total Sulfite (SO22-) in
beverages, foodstuffs and other materials

                      (sulfite oxidase)
(1) SO32- + O2 + H2O ↔ SO42- + H2O2

                         (NADH - peroxidase)
(2) H2O2 + NADH + H+ → 2H2O + NAD+

Kit size:                             * 50 assays (manual) / 500 (microplate)
                                           / 588 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM 
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Total assay time:              ~ 30 min
Detection limit:                 0.3 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, fruit and vegetables,
pharmaceuticals and other materials
Method recognition:    
Methods based on this principle have been accepted by DIN, EN,


  • Very cost effective
  • All reagents stable for > 2 years during use
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.