Trehalase (prokaryote) 

High purity Trehalase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH37
CAS: 9025-52-9

alpha,alpha-trehalase; alpha,alpha-trehalose glucohydrolase

Recombinant. Highly purified. From a prokaryotic source.
In 3.2 M ammonium sulphate.
Supplied at ~ 4,200 U/mL. 

Specific activity:
~ 300 U/mg (40oC, pH 5.5 on trehalose).

Stability: > 4 years at 4oC.

Product Code
8,400 Units

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Trehalase (prokaryote)

CAZy Family: GH37
CAS: 9025-52-9

alpha,alpha-trehalase; alpha,alpha-trehalose glucohydrolase

In 3.2 M ammonium sulphate.

> 4 years at 4oC.

Specific activity:
~ 300 U/mg (40oC, pH 5.5 on trehalose).

Unit definition:
One Unit of trehalase activity is defined as the amount of enzyme required to release two μmoles of D-glucose per minute from trehalose (5 mg/mL) in sodium maleate buffer (100 mM), pH 5.5 at 40oC.

Catalyses the reaction:
Trehalose + H2O = β-D-glucose + α-D-glucose

Applications established for the measurement of trehalose in the food, fermentation, wine, beverage and dairy industries.

The role of oxygen in yeast metabolism during high cell density brewery fermentations.

Verbelen, P. J., Saerens, S. M. G., Van Mulders, S. E., Delvaux, F. & Delvaux, F. R. (2009). Applied Microbiology and Biotechnology, 82(6), 1143-1156.

Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri.

Liverani, S., Mahlow, S., Bouget, F. Y., Pohnert, G., & Sasso, S. (2017). Metabolomics, 13, 68.