Trehalase (prokaryote) 

High purity Trehalase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.28
CAZy Family: GH37
CAS: 9025-52-9

alpha,alpha-trehalase; alpha,alpha-trehalose glucohydrolase

Recombinant. Highly purified. From a prokaryotic source.
In 3.2 M ammonium sulphate.
Supplied at ~ 4,200 U/mL. 

Specific activity:
~ 300 U/mg (40oC, pH 5.5 on trehalose).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-TREH
8,400 Units
$147.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

Trehalase (prokaryote)

EC 3.2.1.28
CAZy Family: GH37
CAS: 9025-52-9

Synonyms:
alpha,alpha-trehalase; alpha,alpha-trehalose glucohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 300 U/mg (40oC, pH 5.5 on trehalose).

Unit definition:
One Unit of trehalase activity is defined as the amount of enzyme required to release two μmoles of D-glucose per minute from trehalose (5 mg/mL) in sodium maleate buffer (100 mM), pH 5.5 at 40oC.

Specificity:
Catalyses the reaction:
Trehalose + H2O = β-D-glucose + α-D-glucose

Applications:
Applications established for the measurement of trehalose in the food, fermentation, wine, beverage and dairy industries.

The role of oxygen in yeast metabolism during high cell density brewery fermentations.

Verbelen, P. J., Saerens, S. M. G., Van Mulders, S. E., Delvaux, F. & Delvaux, F. R. (2009). Applied Microbiology and Biotechnology, 82(6), 1143-1156.

Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri.

Liverani, S., Mahlow, S., Bouget, F. Y., Pohnert, G., & Sasso, S. (2017). Metabolomics, 13, 68.