Trehalose Assay Kit

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

Product Code
100 assays (manual) / 1000 assays (microplate)
/ 1100 assays (auto-analyser)

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UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials

(1) Trehalose + H2O → 2 x D-glucose

(2) D-Glucose + ATP → G-6-P + ADP

     (glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                              * 100 assays (manual) / 1000 (microplate)
                                            / 1100 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved. 
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min
Detection limit:                 37.5 mg/L
Application examples:
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:     Novel method


  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

Improvement of tolerance to freeze–thaw stress of baker’s yeast by cultivation with soy peptides.

Izawa, S., Ikeda, K., Takahashi, N. & Inoue, Y. (2007). Applied Microbiology and Biotechnology, 75(3), 533-537.

Freezing-induced uptake of disaccharides for preservation of chromatin in freeze-dried stallion sperm during accelerated aging.

Oldenhof, H., Zhang, M., Narten, K., Bigalk, J., Sydykov, B., Wolkers, W. F. & Sieme, H. (2017). Biology of Reproduction, 7(6), 892-901.

Starvation resistance and effects of diet on energy reserves in a predatory ground beetle (Merizodus soledadinus; Carabidae) invading the Kerguelen Islands.

Laparie, M., Larvor, V., Frenot, Y. & Renault, D. (2012). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 161(2), 122-129.

Freezing tolerance and low molecular weight cryoprotectants in an invasive parasitic fly, the deer ked (Lipoptena cervi).

Nieminen, P., Paakkonen, T., Eerilä, H., Puukka, K., Riikonen, J., Lehto, V. P. & Mustonen, A. M. (2012). Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 317(1), 1-8.

A novel steamed bread making process using salt‐stressed baker’s yeast.

Yeh, L. T., Wu, M. L., Charles, A. L. & Huang, T. C. (2009). International Journal of Food Science & Technology, 44(12), 2637-2643.

Differences in cold and drought tolerance of high arctic and sub-arctic populations of Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola).

Bahrndorff, S., Petersen, S. O., Loeschcke, V., Overgaard, J. & Holmstrup, M. (2007). Cryobiology, 55(3), 315-323.

Trehalose promotes the survival of Saccharomyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress.

Bandara, A., Fraser, S., Chambers, P. J. & Stanley, G. A. (2009). FEMS Yeast Research, 9(8), 1208-1216.

Divergent strategies for adaptation to desiccation stress in two Drosophila species of immigrans group.

Parkash, R., Aggarwal, D. D., Ranga, P. & Singh, D. (2012). Journal of Comparative Physiology B, 182(6), 751-769.

Sex-specific differences in the physiological basis of water conservation of Drosophila hydei from the western Himalayas.

Parkash, R., Singh, D. & Lambhod, C. (2014). Canadian Journal of Zoology, 92(6), 545-555.

Divergent strategy for adaptation to drought stress in two sibling species of montium species subgroup: Drosophila kikkawai and Drosophila leontia.

Ramniwas, S. & Kajla, B. (2012). Journal of Insect Physiology, 58(12), 1525-1533.

Rapid effects of humidity acclimation on stress resistance in Drosophila melanogaster.

Aggarwal, D. D., Ranga, P., Kalra, B., Parkash, R., Rashkovetsky, E. & Bantis, L. E. (2013). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 166(1), 81-90.

Laser induced injury caused hyperglycemia-like effect in Drosophila larva: a possible insect model for posttraumatic diabetes.

Okabe, F., Nakagiri, Y. & Yamada, T. (2015). Journal of Veterinary Medical Science, 77(5), 601-604.

Targeted mutagenesis and functional analysis of adipokinetic hormone-encoding gene in Drosophila.

Sajwan, S., Sidorov, R., Stašková, T., Žaloudíková, A., Takasu, Y., Kodrík, D. & Zurovec, M. (2015). Insect Biochemistry and Molecular Biology, 61, 79-86.

Use of Drosophila as an Evaluation Method Reveals imp as a Candidate Gene for Type 2 Diabetes in Rat Locus Niddm22.

Kawasaki, K., Yamada, S., Ogata, K., Saito, Y., Takahama, A., Yamada, T., Matsumoto, K. & Kose, H. (2015). Journal of Diabetes Research, 2015, Article ID 758564.

Short-term adaptation during propagation improves the performance of xylose-fermenting Saccharomyces cerevisiae in simultaneous saccharification and co-fermentation.

Nielsen, F., Tomás-Pejó, E., Olsson, L. & Wallberg, O. (2015). Biotechnology for Biofuels, 8(1), 219.

Effect of C-terminal domain truncation of Thermus thermophilus trehalose synthase on its substrate specificity.

Cho, C. B., Park, D. Y. & Lee, S. B. (2017). Enzyme and Microbial Technology, 96, 121-126.

Cold and desiccation stress induced changes in the accumulation and utilization of proline and trehalose in seasonal populations of Drosophila immigrans.

Tamang, A. M., Kalra, B. & Parkash, R. (2017). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 203, 304-313.

Thermoresponsive microgels containing trehalose as soft matrices for 3D cell culture.

Burek, M., Waśkiewicz, S., Lalik, A., Student, S., Bieg, T. & Wandzik, I. (2017). Biomaterials Science, 5(2), 234-246.

Adipokinetic hormone activities in insect body infected by entomopathogenic nematode.

Ibrahim, E., Hejníková, M., Shaik, H. A., Doležel, D. & Kodrík, D. (2017). Journal of Insect Physiology, 98, 347-355.

Freezing-induced uptake of trehalose into mammalian cells facilitates cryopreservation.

Zhang, M., Oldenhof, H., Sieme, H. & Wolkers, W. F. (2016). Biochimica et Biophysica Acta (BBA)-Biomembranes, 1858(6), 1400-1409.

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