Xylanase Assay Kit (Azo-Wax) 

The Xylanase (Azo-Wax) test kit is suitable for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Containing Azo-wheat arabinoxylan and a Trichoderma sp. xylanase control.

Product Code
200 assays per kit

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

Colourimetric method for the determination of Xylanase in feed,
foodstuffs and other materials

(1)       Azo-WAX + H2O         →              Azo-WAX fragments
     (insoluble in aqueous alcohol)        (soluble in aqueous alcohol)

Kit size:                              200 assays
Method:                             Based on use of Azo-WAX reagent (590 nm)
Total assay time:              ~ 45 min
Detection limit:                 0.2 U/mL of assay solution
Application examples:
Animal feeds, enzyme preparations, bread improver mixtures and
other materials
Method recognition:    
Used widely in the feed industry


  • Very cost effective
  • All reagents stable for > 2 years
  • Only test kit available
  • Simple format
  • Standard included

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Comparison of endolytic hydrolases that depolymerize 1,4-β-D-mannan, 1,5-α-L-arabinan and 1,4-β-D-galactan.

McCleary, B. V. (1991). “Enzymes in Biomass Conversion”, (M. E. Himmel and G. F. Leatham, Eds.), ACS Symposium Series 460, Chapter 34, pp. 437-449. American Chemical Society, Washington.

Measurement of endo-1,4-β-D-xylanase.

McCleary, B. V. (1992). “Xylans and Xylanases”, (J. Visser, G. Beldman, M. A. Kusters-van Someron and A. G. J. Voragen, Eds.), Progress in Biotechnology, Vol. 7, Elsevier, Science Publishers B. V., pp. 161-169.

New developments in the measurement of α-amylase, endo-protease, β-glucanase and β-xylanase.

McCleary, B. V. & Monaghan, D. (2000). “Proceedings of the Second European Symposium on Enzymes in Grain Processing”, (M. Tenkanen, Ed.), VTT Information Service, pp. 31-38.

Analysis of feed enzymes.

McCleary, B. V. (2001). “Enzymes in Farm Animal Nutrition”, (M. Bedford and G. Partridge, Eds.), CAB International, pp. 85-107.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, September, 398-401.

Optimising the response.

Acamovic, T. & McCleary, B. V. (1996). Feed Mix, 4, 14-19.

Xylanase B from the hyperthermophile Thermotoga maritima as an indicator for temperature gradients in high pressure high temperature processing.

Vervoort, L., Van der Plancken, I., Grauwet, T., Verjans, P., Courtin, C. M., Hendrickx, M. E. & Van Loey, A. (2011). Innovative Food Science & Emerging Technologies, 12(2), 187-196.

Identification of multiple highly similar XIP-type xylanase inhibitor genes in hexaploid wheat.

Takahashi-Ando, N., Inaba, M., Ohsato, S., Igawa, T., Usami, R. & Kimura, M. (2007). Biochemical and Biophysical Research Communications, 360(4), 880-884.

Sprouted wheat as an alternative to conventional flour improvers in bread-making.

Marti, A., Cardone, G., Nicolodi, A., Quaglia, L. & Pagani, M. A. (2017). LWT-Food Science and Technology, 80, 230-236.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.