Xylanase Assay Kit (Xylazyme AX) 

The Xylanase (Xylazyme AX) test kit is used for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Contains Xylazyme AX Tablets and xylanase enzyme controls (A. niger and Trichoderma longibrachiatum).

Product Code
200 assays per kit

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Colourimetric method for the determination of Xylanase in feed,
foodstuffs and other materials

(1) Xylazyme AX (water insoluble) + H2O → water soluble dyed xylan fragments

Kit size:                           200 assays
Method:                            Based on use of Xylazyme AX tablets (590 nm)
Total assay time:              ~ 30 min
Detection limit:                 0.02 U/mL of assay solution
Application examples:
Animal feeds, enzyme preparations, bread improver mixtures and
other materials
Method recognition:    
Used widely in the feed industry


  • Very cost effective
  • All reagents stable for > 2 years during use
  • Only test kit available
  • Simple format
  • Standard included

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Comparison of endolytic hydrolases that depolymerise 1,4-β-D-mannan, 1,5-α-L-arabinan and 1,4-β-D-galactan.

McCleary, B. V. (1991). “Enzymes in Biomass Conversion”, (M. E. Himmel and G. F. Leatham, Eds.), ACS Symposium Series, 460, Chapter 34, pp. 437-449. American Chemical Society, Washington.

Measurement of endo-1,4-β-D-xylanase.

McCleary, B. V. (1992). “Xylans and Xylanases”, (J. Visser, G. Beldman, M. A. Kusters-van Someron and A. G. J. Voragen, Eds.), Progress in Biotechnology, Vol. 7, Elsevier, Science Publishers B. V., pp. 161-169.

New developments in the measurement of α-amylase, endo-protease, β-glucanase and β-xylanase.

McCleary, B. V. & Monaghan, D. (2000). “Proceedings of the Second European Symposium on Enzymes in Grain Processing”, (M. Tenkanen, Ed.), VTT Information Service, pp. 31-38.

Analysis of feed enzymes.

McCleary, B. V. (2001). “Enzymes in Farm Animal Nutrition”, (M. Bedford and G. Partridge, Eds.), CAB International, pp. 85-107.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, September, 398-401.

Optimising the response.

Acamovic, T. & McCleary, B. V. (1996). Feed Mix, 4, 14-19.

Comparative study of colorectal health related compounds in different types of bread: Analysis of bread samples pre and post digestion in a batch fermentation model of the human intestine.

Hiller, B., Schlörmann, W., Glei, M. & Lindhauer, M. G. (2011). Food chemistry, 125(4), 1202-1212.

Standard assays do not predict the efficiency of commercial cellulase preparations towards plant materials.

Kabel, M. A., Van der Maarel, M. J. E. C., Klip, G., Voragen, A. G. J. & Schols, H. A. (2006). Biotechnology and Bioengineering, 93(1), 56-63.

Characteristics of pregelatinized AE mutant rice flours prepared by boiling after preroasting.

Nakamura, S., Satoh, H. & Ohtsubo, K. I. (2011). Journal of Agricultural and Food Chemistry, 59(19), 10665-10676.

Variability in Arabinoxylan, Xylanase Activity, and Xylanase Inhibitor Levels in Hard Spring Wheat.

Mendis, M., Ohm, J. B., Delcour, J. A., Gebruers, K., Meinhardt, S. & Simsek, S. (2013). Cereal Chemistry, 90(3), 240-248.

Wheat dough syruping in cold storage is related to structural changes of starch and non-starch polysaccharides.

Kim, H. J., Song, Y., Lee, S., Lee, K. P., Lee, B. H. & Yoo, S. H. (2017). Food Research International, 99, 596-602.

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.