Xylazyme AX Tablets

High purity dyed and crosslinked Xylazyme AX (60 mg tablets) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,4-β-D-xylanase. Containing AZCL-arabinoxylan (wheat).

Xylazyme AX Tablets
Product Code
1,000 Tablets
200 Tablets

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Validation of Methods

RACI Standard Method

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Comparison of endolytic hydrolases that depolymerise 1,4-β-D-mannan, 1,5-α-L-arabinan and 1,4-β-D-galactan.

McCleary, B. V. (1991). “Enzymes in Biomass Conversion”, (M. E. Himmel and G. F. Leatham, Eds.), ACS Symposium Series 460, Chapter 34, pp. 437-449. American Chemical Society, Washington.

Measurement of endo-1,4-β-D-xylanase.

McCleary, B. V. (1992). “Xylans and Xylanases”, (J. Visser, G. Beldman, M. A. Kusters-van Someron and A. G. J. Voragen, Eds.), Progress in Biotechnology Vol. 7, Elsevier, Science Publishers B. V., pp. 161-169.

Measurement of polysaccharide degrading enzymes using chromogenic and colorimetric substrates.

McCleary, B. V. (1991). Chemistry in Australia, 58, 398-401.

Optimising the response.

Acamovic, T. & McCleary, B. V. (1996). Feed Mix, 4, 14-19.

Cloning and characterization of arabinoxylan arabinofuranohydrolase-D3 (AXHd3) from Bifidobacterium adolescentis DSM20083.

Van den Broek, L. A. M., Lloyd, R. M., Beldman, G., Verdoes, J. C., McCleary, B. V. & Voragen, A. G. J. (2005). Applied Microbiology and Biotechnology, 67(5), 641-647.

Recombinant expression and characterization of XynD from Bacillus subtilis subsp. subtilis ATCC 6051: a GH 43 arabinoxylan arabinofuranohydrolase.

Bourgois, T. M., Van Craeyveld, V., Van Campenhout, S., Courtin, C. M., Delcour, J. A., Robben, J. & Volckaert, G. (2007). Applied Microbiology and Biotechnology, 75(6), 1309-1317.

Variability in the release of free and bound hydroxycinnamic acids from diverse malted barley (Hordeum vulgare L.) cultivars during wort production.

Vanbeneden, N., Gils, F., Delvaux, F. & Delvaux, F. R. (2007). Journal of Agricultural and Food Chemistry, 55(26), 11002-11010.

Variability in the structure of rye flour alkali-extractable arabinoxylans.

Verwimp, T., Van Craeyveld, V., Courtin, C. M. & Delcour, J. A. (2007). Journal of Agricultural and Food Chemistry, 55(5), 1985-1992.

Impact of wheat flour-associated endoxylanases on arabinoxylan in dough after mixing and resting.

Dornez, E., Gebruers, K., Cuyvers, S., Delcour, J. A. & Courtin, C. M. (2007). Journal of Agricultural and Food Chemistry, 55(17), 7149-7155.

An accurate normalization strategy for RT-qPCR in Hypocrea jecorina (Trichoderma reesei).

Steiger, M. G., Mach, R. L. & Mach-Aigner, A. R. (2010). Journal of Biotechnology, 145(1), 30-37.

Xylanases from microbial origin induce syrup formation in dough.

De Schryver, P., Sesena, S., Decaigny, B., Van de Wiele, T., Verstraete, W. & Boon, N. (2008). Journal of Cereal Science, 47(1), 18-28.

Transcriptional regulation of xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina.

Mach-Aigner, A. R., Pucher, M. E., Steiger, M. G., Bauer, G. E., Preis, S. J. & Mach, R. L. (2008). Applied and Environmental Microbiology, 74(21), 6554-6562.

A screening method for endo-β-1,4-xylanase substrate selectivity.

Moers, K., Courtin, C. M., Brijs, K. & Delcour, J. A. (2003). Analytical Biochemistry, 319(1), 73-77.

Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina.

Stricker, A., R., Steiger, M., G. & Mach, R., L. (2007). FEBS Letters, 581(21), 3915-3920.

Multi-carbohydrase and phytase supplementation improves growth performance and liver insulin receptor sensitivity in broiler chickens fed diets containing full-fat rapeseed.

Józefiak, D., Ptak, A., Kaczmarek, S., Maćkowiak, P., Sassek, M. & Slominski, B. A. (2010). Poultry Science, 89(9), 1939-1946.

Partial purification of components in rye water extractables which improve the quality of oat bread.

Pauly, A. & Delcour, J. A. (2018). Journal of Cereal Science, 79, 141-147.

Impact of water-extractable components from different cereals on the quality of oat bread.

Pauly, A. & Delcour, J. A. (2018). Journal of Cereal Science, 79, 134-140.
Analysis of enzymes activity using carbohydrase tablet testing

To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Theory of endo-1, 4-Beta-D-Xylanase Assay Procedure
Chapter 2: Buffers & Reagents
Chapter 3: Assay Procedure

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.