Xyloglucanase (GH5) (Paenibacillus sp.) 

High purity recombinant Xyloglucanase (GH5) (Paenibacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH5
CAS: 76901-10-5

xyloglucan-specific endo-beta-1,4-glucanase; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase

Recombinant. From Paenibacillus sp.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity:
~ 70 U/mg (40oC, pH 5.5 on tamarind xyloglucan).

Stability: > 2 years at 4oC. 

Product Code
3,000 Units

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Xyloglucanase (Paenibacillus sp.)

CAZy Family: GH5
CAS: 76901-10-5

xyloglucan-specific endo-beta-1,4-glucanase; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase

In 3.2 M ammonium sulphate.

> 2 years at 4oC.

Specific activity:
~ 70 U/mg (40oC, pH 5.5 on tamarind xyloglucan).

Unit definition:
One Unit of xyloglucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from xyloglucan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.5 at 40oC. 

endo-hydrolysis of 1,4-β-D-glucosidic linkages in xyloglucan.

Applications in carbohydrate and biofuels research.

A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

Park, S., Szumlanski, A. L., Gu, F., Guo, F. & Nielsen, E. (2011). Nature Cell Biology, 13(8), 973-980.

The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific.

Hu, J., Arantes, V., Pribowo, A. & Saddler, J. N. (2013). Biotechnology for Biofuels, 6(1), 112.

Identification of quantitative trait loci affecting hemicellulose characteristics based on cell wall composition in a wild and cultivated rice species.

Zhang, S. J., Song, X. Q., Yu, B. S., Zhang, B. C., Sun, C. Q., Knox, J. P. & Zhou, Y. H. (2012). Molecular Plant, 5(1), 162-175.

XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in Arabidopsis.

Zhu, W. X. F., Shi, Y. Z., Lei, G. J., Fry, S. C., Zhang, B. C., Zhou, Y. H., Braam, J., Jiang, T., Xu, X. Y., Mao, C. Z., Pan, Y. J., Yang, J. L., Wu, P. & Zheng, S. J. (2012). The Plant Cell, 24(11), 4731-4747.

Profiling the main cell wall polysaccharides of tobacco leaves using high-throughput and fractionation techniques.

Nguema-Ona, E., Moore, J. P., Fagerström, A., Fangel, J. U., Willats, W. G. T., Hugoc, A. & Vivier, M. A. (2012). Carbohydrate Polymers, 88(3), 939-949.