Xyloglucanase (GH5) (Paenibacillus sp.) 

High purity recombinant Xyloglucanase (GH5) (Paenibacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH5
CAS: 76901-10-5

xyloglucan-specific endo-beta-1,4-glucanase; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase

Recombinant. From Paenibacillus sp.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity:
~ 70 U/mg (40oC, pH 5.5 on tamarind xyloglucan).

Stability: Minimum 1 year at 4oC. Check vial for details.

Product Code
3,000 Units

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page


Xyloglucanase (Paenibacillus sp.)

CAZy Family: GH5
CAS: 76901-10-5

xyloglucan-specific endo-beta-1,4-glucanase; [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase

In 3.2 M ammonium sulphate.

Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 70 U/mg (40oC, pH 5.5 on tamarind xyloglucan).

Unit definition:
One Unit of xyloglucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from xyloglucan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.5 at 40oC. 

endo-hydrolysis of 1,4-β-D-glucosidic linkages in xyloglucan.

Applications in carbohydrate and biofuels research.

A role for CSLD3 during cell-wall synthesis in apical plasma membranes of tip-growing root-hair cells.

Park, S., Szumlanski, A. L., Gu, F., Guo, F. & Nielsen, E. (2011). Nature Cell Biology, 13(8), 973-980.

The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific.

Hu, J., Arantes, V., Pribowo, A. & Saddler, J. N. (2013). Biotechnology for Biofuels, 6(1), 112.

Profiling the main cell wall polysaccharides of tobacco leaves using high-throughput and fractionation techniques.

Nguema-Ona, E., Moore, J. P., Fagerström, A., Fangel, J. U., Willats, W. G. T., Hugoc, A. & Vivier, M. A. (2012). Carbohydrate Polymers, 88(3), 939-949.

Identification of quantitative trait loci affecting hemicellulose characteristics based on cell wall composition in a wild and cultivated rice species.

Zhang, S. J., Song, X. Q., Yu, B. S., Zhang, B. C., Sun, C. Q., Knox, J. P. & Zhou, Y. H. (2012). Molecular Plant, 5(1), 162-175.

XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in Arabidopsis.

Zhu, W. X. F., Shi, Y. Z., Lei, G. J., Fry, S. C., Zhang, B. C., Zhou, Y. H., Braam, J., Jiang, T., Xu, X. Y., Mao, C. Z., Pan, Y. J., Yang, J. L., Wu, P. & Zheng, S. J. (2012). The Plant Cell, 24(11), 4731-4747.

Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module.

Hernandez-Gomez, M. C., Rydahl, M. G., Rogowski, A., Morland, C., Cartmell, A., Crouch, L., Labourel, A., Fontes, C. M. G. A., Willats, W. G. T., Gilbert, H. J. & Knox, J. P. (2015). FEBS Letters, 589(18), 2297-2303.

Methods for Xyloglucan Structure Analysis in Brachypodium distachyon.

Liu, L. (2017). Brachypodium Genomics, Humana Press, New York, NY, 65-71.

Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-β-Xylanase from Caldicellulosiruptor owensensis.

Liu, X., Liu, T., Zhang, Y., Xin, F., Mi, S., Wen, B., Gu, T., Xinyuan Shi, X., Wang, F. & Sun, L. (2017). Journal of agricultural and food chemistry, 66(1), pp 187-193.

Xyloglucan Fucosylation Modulates Arabidopsis Cell Wall Hemicellulose Aluminium binding Capacity.

Wan, J. X., Zhu, X. F., Wang, Y. Q., Liu, L. Y., Zhang, B. C., Li, G. X., Zhou, Y. H. & Zheng, S. J. (2018). Scientific Reports, 8(1), 428.