Xylose dehydrogenase + Xylose mutarotase

High purity recombinant Xylose dehydrogenase + Xylose mutarotase for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

(EC 1.1.1.175) XDH
CAS: 62931-20-8

(EC 5.1.3.3) XMR
CAS: 9031-76-9

(XDH): D-xylose 1-dehydrogenase; D-xylose:NAD+ 1-oxidoreductase
(XMR): Aldose 1-epimerase

Recombinant.
In 50% (v/v) glycerol.
Supplied at ~ 60 U/mL. 

Activity/Concentration:
XDH: ~ 60 U/mL at pH 7.5 and 25oC / XMR: 2 mg/mL

Stability: > 2 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-XYLMUT
2.5 mL; xylose dehydrogenase (60 U/mL) / xylose mutarotase (2 mg/mL)
$191.00

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DESCRIPTION

Xylose dehydrogenase + Xylose mutarotase

(EC 1.1.1.175) XDH
CAS: 62931-20-8

(EC 5.1.3.3) XMR
CAS: 9031-76-9 

Synonyms
(XDH): D-xylose 1-dehydrogenase; D-xylose:NAD+ 1-oxidoreductase
(XMR): Aldose 1-epimerase 

Form:
In 50% (v/v) glycerol.

Stability:
> 2 years at -20oC. 

Activity/Concentration:
Xylose dehydrogenase: ~ 60 U/mL at pH 7.5 and 25oC.
Xylose mutarotase: 2 mg/mL 

Unit definition:
One Unit of xylose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute at 25oC. 

Specificity:
Interconversion of the α- and β-anomeric forms of D-xylose is catalysed by xylose mutarotase (XMR) (1). 

                     (XMR)
(1)  α-D-Xylose  ↔  β-D-xylose

The β-D-xylose is oxidised by NAD+ to D-xylonic acid in the presence of β-xylose dehydrogenase (β-XDH) at pH 7.5 (2). 

                                (β-XDH)
(2)  β-D-Xylose + NAD+ ↔ D-xylonic acid + NADH + H+

Applications:
For the measurement of D-xylose, especially in broths and hydrolysates of plant material and polysaccharides. Refer to the D-XYLOSE Assay Kit booklet (Megazyme cat. no. K-XYLOSE) for directions of use.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.