Enzymatic Yeast β-Glucan Assay Kit

Enzymatic Yeast Beta-Glucan test kit, an enzymatic procedure for the measurement and analysis of 1,3:1,6-β-glucan in yeast. Also measures 1,3-β-glucan.

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Product Code
50 assays per kit

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Colourimetric method for the enzymatic determination of
yeast β-Glucan and also (1-3)-β Glucans

                                                     (KOH, 4°C, 30 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + H2O → soluble glucan

                      (glucazyme, 40°C, 16 h)
(2) Soluble glucan + H2O → D-glucose

                            (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                        quinoneimine + 4H2O

Kit size:                            50 assays
Method:                            Spectrophotometric at 510 nm
Reaction time:                  ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations and other materials
Method recognition:       Novel method


  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

In Vitro potential antioxidant activity of (1→ 3),(1→ 6)-β-D-glucan and protein fractions from Saccharomyces cerevisiae cell walls.

Jaehrig, S. C., Rohn, S., Kroh, L. W., Fleischer, L. G. & Kurz, T. (2007). Journal of Agricultural and Food Chemistry, 55(12), 4710-4716.

Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.

Walachowski, S., Tabouret, G. & Foucras, G. (2016). PloS one, 11(2), e0148464.

Quantification of 1,3-β-D-glucan from yeast added as a functional ingredient to bread.

Rieder, A., Ballance, S., Böcker, U. & Knutsen, S. (2017). Carbohydrate Polymers, 181, 34-42.

Isolation of Functional Components β-Glucan and γ-Amino Butyric Acid from Raw and Germinated Barnyard Millet (Echinochloa frumentaceae) and their Characterization.

Sharma, S., Saxena, D. C. & Riar, C. S. (2016). Plant Foods for Human Nutrition, 71(3), 231-238.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Theory of the Yeast Beta Glucan Assay Procedure
Chapter 2: Kit Components
Chapter 3: Preparation of Reagents
Chapter 4: Preparation of other Reagents required
Chapter 5: Measurement of 1, 3:1, 6-Beta-Glucan in Yeast Preparations
Chapter 6: Calculations

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