Enzymatic Yeast β-Glucan Assay Kit

Enzymatic Yeast Beta-Glucan test kit, an enzymatic procedure for the measurement and analysis of 1,3:1,6-β-glucan in yeast. Also measures 1,3-β-glucan.

Image unavailable
Product Code
Content/size
Stock
Price
Qty
K-EBHLG
50 assays per kit
$288.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

Colourimetric method for the enzymatic determination of
yeast β-Glucan and also (1-3)-β Glucans

Principle:
                                                     (KOH, 4°C, 30 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + H2O → soluble glucan

                      (glucazyme, 40°C, 16 h)
(2) Soluble glucan + H2O → D-glucose

                            (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

                                                                                  (peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
                                                                        quinoneimine + 4H2O

Kit size:                            50 assays
Method:                            Spectrophotometric at 510 nm
Reaction time:                  ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations and other materials
Method recognition:       Novel method

Advantages

  • Very competitive price (cost per test)
     
  • All reagents stable for > 12 months after preparation
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Standard included

In Vitro potential antioxidant activity of (1→ 3),(1→ 6)-β-D-glucan and protein fractions from Saccharomyces cerevisiae cell walls.

Jaehrig, S. C., Rohn, S., Kroh, L. W., Fleischer, L. G. & Kurz, T. (2007). Journal of Agricultural and Food Chemistry, 55(12), 4710-4716.

Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages.

Walachowski, S., Tabouret, G. & Foucras, G. (2016). PloS one, 11(2), e0148464.

Quantification of 1,3-β-D-glucan from yeast added as a functional ingredient to bread.

Rieder, A., Ballance, S., Böcker, U. & Knutsen, S. (2017). Carbohydrate Polymers, 181, 34-42.
To choose a chapter, play the video and select the required chapter from the options on the video display.

Chapter 1: Theory of the Yeast Beta Glucan Assay Procedure
Chapter 2: Kit Components
Chapter 3: Preparation of Reagents
Chapter 4: Preparation of other Reagents required
Chapter 5: Measurement of 1, 3:1, 6-Beta-Glucan in Yeast Preparations
Chapter 6: Calculations

Below you will find a link to our dedicated frequently asked questions section. Within this section you will find common questions and answers on a range of topics about the product.

FAQs