α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima)

High purity α-Mannosidase (Thermotoga maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Recombinant. From Thermotoga maritima.
In 3.2 M ammonium sulphate.
Supplied at ~ 71 U/mL. 

Specific activity:
4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC;
~ 0.2 U/mg at 40oC.

Stability: > 4 years 4oC.

Product Code
Content/size
Stock
Price
Qty
E-AMANTM
5 Units at 90oC
$185.00

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DESCRIPTION

α-(1-2,3,4,6)-D-Mannosidase (Thermotoga maritima)

EC 3.2.1.24
CAZy Family: GH38
CAS: 9025-42-7

Synonyms:
alpha-mannosidase; alpha-D-Mannoside mannohydrolase, alpha-D-mannopyranosidase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years 4oC.

Specific activity:
4.5 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.0 and 90oC;
~ 0.2 U/mg at 40oC.

Unit definition:
One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-mannopyranoside (2 mM) in MES buffer (100 mM) and CoCl2 (1 mM) at pH 6.0 at the temperatures indicated.

Specificity:
Broad specificity; hydrolysis of terminal non-reducing alpha-D-mannose in alpha-D-mannosides.

Applications:
For use in glycobiology research.