α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.51
CAZy Family: GH29
CAS: 9037-65-4

alpha-L-fucosidase; alpha-L-fucoside fucohydrolase

Recombinant. From Homo sapiens.
In 3.2 M ammonium sulphate.
Supplied at ~ 50 U/mL.

Specific activity:
7 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 25oC;
~ 23 U/mg at 37oC;
~ 68 U/mg at 50oC.

Stability: Minimum 1 year at 4oC. Check vial for details.

DESCRIPTION

α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens)

EC 3.2.1.51
CAZy Family: GH29
CAS: 9037-65-4

Synonyms:
alpha-L-fucosidase; alpha-L-fucoside fucohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
Minimum 1 year at 4oC. Check vial for details.

Specific activity:
7 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 25oC;
~ 23 U/mg at 37oC;
~ 68 U/mg at 50oC.

Unit definition:
One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-fucopyranoside (1 mM) in sodium acetate buffer (100 mM) at pH 4.0 at the temperatures indicated.

Specificity:
Broad specificity; hydrolysis of terminal non-reducing α-(1-2,3,4,6)-linked L-fucose residues from glycoproteins and oligosaccharides.

Applications:
For use in glycobiology research.