α-L-Arabinofuranosidase (Aspergillus nidulans)

High purity α-L-arabinofuranosidase (Aspergillus nidulans) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.55
CAZy Family: GH62
CAS: 9067-74-7

non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase; Arabinoxylan arabinofuranohydrolase axhA-2

Recombinant. From Aspergillus nidulans.
In 3.2 M ammonium sulphate.
Supplied at ~ 500 U/mL. 

Specific activity:
~ 89 U/mg protein (on wheat arabinoxylan) at pH 4.5 and 40oC.

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-ABFAN
500 Units
$185.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

α-L-Arabinofuranosidase (Aspergillus nidulans)

EC 3.2.1.55
CAZy Family: GH62
CAS: 9067-74-7

Synonyms:
non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase; Arabinoxylan arabinofuranohydrolase axhA-2

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC

Specific activity:
~ 89 U/mg protein (on wheat arabinoxylan) at pH 4.5 and 40oC.

Unit definition:
One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of arabinose per minute from wheat arabinoxylan (10 mg/ mL) in sodium acetate (100 mM) at pH 4.5 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing α-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (α-1,2 > α-1,3).  Does not hydrolyse α-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.

Applications:
For use in plant cell wall carbohydrate and biofuels research.

An efficient arabinoxylan-debranching α-L-arabinofuranosidase of family GH62 from Aspergillus nidulans contains a secondary carbohydrate binding site.

Wilkens, C., Andersen, S., Petersen, B. O., Li, A., Busse-Wicher, M. B., Birch, J., Cockburn, D., Nakai, H., Christensen, H. E. M., Kragelund, B. B., Dupree, P., McCleary, B., Hindsgaul, O., Hachem, M. A. & Svensson, B. (2016). Applied Microbiology and Biotechnology, 100(14), 6265–6277.