α-L-Arabinofuranosidase B17 (Bacteroides ovatus)

High purity α-L-arabinofuranosidase (Bacteroides ovatus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.55
CAZy Family: GH43
CAS: 9067-74-7

non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

Recombinant. From Bacteroides ovatus.
In 50% Glycerol.
Supplied at ~ 1,000 U/mL. 

Specific activity: 
~ 575 U/mg protein (on wheat arabinoxylan) at pH 6.5 and 40oC.

Stability: > 4 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-ABFBO17
2,000 Units
$185.00

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DESCRIPTION

α-L-Arabinofuranosidase B17 (Bacteroides ovatus)

EC 3.2.1.55
CAZy Family: GH43
CAS: 9067-74-7

Synonyms:
non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

Form:
In 50% Glycerol.

Stability: 
> 4 years -20oC.

Specific activity:
~ 575 U/mg protein (on wheat arabinoxylan) at pH 6.5 and 40oC.

Unit definition:
One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium phosphate (100 mM) at pH 7.5 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing α-1,3 linked L-arabinofuranose residues from doubly substituted xylose residues in arabinoxylan. Does not hydrolyse α-L-arabinofuranose from singly substituted xylose residues in arabinoxylan.

Applications:
For use in plant cell wall carbohydrate and biofuels research.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

Glycan complexity dictates microbial resource allocation in the large intestine.

Rogowski, A., Briggs, J. A., Mortimer, J. C., Tryfona, T., Terrapon, N., Lowe, E. C., Baslé, A., Morland, C., Day, A. M., Zheng, H., Rogers, T. E., Thompson, P., Hawkins, A. R., Yadav, M. P., Henrissat, B., Martens, E. C., Dupree, P., Gilbert, H. J. & Bolam, D. N. (2015). Nature Communications, 6, 7481.