α-L-Arabinofuranosidase (Ustilago maydis)

High purity α-L-Arabinofuranosidase (Ustilago maydis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.55
CAZy Family: GH62
CAS: 9067-74-7 

non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

Recombinant. From Ustilago maydis. 
In 3.2 M ammonium sulphate. 
Highly active on wheat arabinoxylan. 
Supplied at ~ 200 U/mL. 

Specific activity:
~ 25 U/mg protein (40oC, pH 5.0 on wheat arabinoxylan).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-ABFUM
400 Units at 40oC
$185.00

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DESCRIPTION

α-L-Arabinofuranosidase (Ustilago maydis)

EC 3.2.1.55
CAZy Family: GH62
CAS: 9067-74-7 

Synonyms:
non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase

Form:
In 3.2 M ammonium sulphate. 

Stability:
> 2 years at 4oC. 

Specific activity:
~ 25 U/mg protein (40oC, pH 5.0 on wheat arabinoxylan)

Unit definition:
One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one μmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC.

Specificity:
Hydrolysis of terminal, non-reducing α-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (α-1,2 > α-1,3).  Does not hydrolyse α-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.

Applications:
For use in plant cell wall carbohydrate and biofuels research.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.