Cellobiohydrolase II (microbial)

High purity Cellobiohydrolase II (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.91
CAZy Family: GH6
CAS: 37329-65-0

cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end)

Recombinant. From microbial source.
In 50% glycerol.
Supplied at ~ 80 U/mL. 

Specific activity:
~ 28 U/mg protein (on 1,4-β-D-cellopentaitol) at pH 5.5 and 40oC.

Stability: > 4 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-CBHIIM
200 Units
$185.00

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DESCRIPTION

Cellobiohydrolase II (microbial)

EC 3.2.1.91
CAZy Family: GH6
CAS: 37329-65-0

Synonyms:
cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end)

Form:
In 50% glycerol.

Stability: 
> 4 years at -20oC.

Specific activity:
~ 28 U/mg protein (on 1,4-β-D-cellopentaitol) at pH 5.5 and 40oC.

Unit definition:
One Unit of cellobiohydrolase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from 1,4-β-D-cellopentaitol (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.5 at the appropriate temperature.

Specificity:
Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellooligosaccharides (DP < 4), releasing cellobiose from the non-reducing ends of the chains.

Applications:
For use in research.