endo-1,3-β-D-Glucanase (barley) (Recombinant)

High purity recombinant endo-1,3-β-Glucanase (barley) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.39
CAZy Family: GH17
CAS: 9025-37-0 

glucan endo-1,3-beta-D-glucosidase; 3-beta-D-glucan glucanohydrolase

Recombinant. From barley (Hordeum vulgare).
In 50% (v/v) glycerol.
Supplied at ~ 2,500 U/mL. 

Specific activity:
~ 100 U/mg (40oC, pH 5.0 on laminarin).

Stability: > 4 years at -20oC.

Product Code
Content/size
Stock
Price
Qty
E-LAMHV
5,000 Units
$185.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon GMT, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page. Should delivery error or damage require you to return a product please contact our Customer Service team to obtain shipping instructions and authorisation. For full terms and conditions see T&Cs.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

endo-1,3-β-D-Glucanase (barley) (Recombinant)

EC 3.2.1.39
CAZy Family: GH17
CAS: 9025-37-0 

Synonyms:
glucan endo-1,3-beta-D-glucosidase; 3-beta-D-glucan glucanohydrolase

Form:
In 50% (v/v) glycerol.

Stability: 
> 4 years at -20oC.

Specific activity:
~ 100 U/mg (40oC, pH 5.0 on laminarin).

Unit definition:
One Unit of endo-1,3-β-D-Glucanase activity is defined as the amount of enzyme required to release one μmole of glucose-reducing-sugar equivalents per minute in the presence of laminarin (10 mg/mL) in sodium acetate buffer (100mM), pH 5.0 at 40oC.

Specificity:
endo-hydrolysis of (1,3)-β-D-glucosidic linkages in (1,3)-β-D-glucans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds industry.

Characterization and comparison of polysaccharides from Lycium barbarum in China using saccharide mapping based on PACE and HPTLC.

Wu, D. T., Cheong, K. L., Deng, Y., Lin, P. C., Wei, F., Lv, X. J., Long, Z. R., Zhoa, J., Ma, S. C. & Li, S. P. (2015). Carbohydrate polymers, 134, 12-19.