endo-1,3-β-D-Glucanase (Trichoderma sp.) 

High purity endo-1,3-β-Glucanase (Trichoderma sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.39
CAZy Family: GH16
CAS: 9025-37-0 

glucan endo-1,3-beta-D-glucosidase; 3-beta-D-glucan glucanohydrolase

Highly purified. From Trichoderma sp.
In 3.2 M ammonium sulphate.
Supplied at ~ 50 U/mL. 

Specific Activity:
~ 15 U/mg (40oC, pH 4.5 on CM-Curdlan).

Stability: > 4 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-LAMSE
100 Units
$182.00

In association with DHL Express Megazyme offers expedited same day shipping on all orders received before 12 noon, DHL offers express shipping to over 220 countries worldwide serving 35 countries next day and 65 within 2 days. For further details visit our delivery page.

We support the following payment methods:

  • Visa
  • MasterCard
  • American Express
  • Cheque
  • Wire Transfer / EFT /ACH

For further details visit our payment page

DESCRIPTION

endo-1,3-β-D-Glucanase (Trichoderma sp.)

EC 3.2.1.39
CAZy Family: GH16

CAS: 9025-37-0

Synonyms:
glucan endo-1,3-beta-D-glucosidase; 3-beta-D-glucan glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 4 years at 4oC.

Specific activity:
~ 15 U/mg (40oC, pH 4.5 on CM-Curdlan).

Unit definition:
One Unit of endo-1,3-β-D-Glucanase activity is defined as the amount of enzyme required to release one μmole of glucose-reducing-sugar equivalents per minute in the presence of CM-Curdlan (5 mg/mL) in sodium acetate buffer (200 mM) at pH 4.5 and 40oC.

Specificity:
Hydrolysis of (1,3)-β-D-glucosidic linkages in (1,3)-β-D-glucans.

Applications:
Applications in carbohydrate and biofuels research and in the food and feeds industry.

Dietary fibers from mushroom sclerotia: 3. In vitro fermentability using human fecal microflora.

Wong, K. H., Wong, K. Y., Kwan, H. S. & Cheung, P. C. K. (2005). Journal of Agricultural and Food Chemistry, 53(24), 9407-9412.

Regulation of the cellulose synthase-like gene family by light in the maize mesocotyl.

Van Erp, H. & Walton, J. D. (2009). Planta, 229(4), 885-897.