endo-1,4-β-Galactanase (Cellvibrio japonicus)

High purity recombinant endo-1,4-β-Galactanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Recombinant. From Cellvibrio japonicus.
In 3.2 M ammonium sulphate.
Supplied at ~ 750 U/mL. 

Specific activity:
~ 100 U/mg (40oC, pH 8.0 on potato galactan).

Stability: > 2 years at 4oC.

Product Code
Content/size
Stock
Price
Qty
E-GALCJ
1,500 Units
$216.00

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DESCRIPTION

endo-1,4-β-Galactanase (Cellvibrio japonicus)

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

Synonyms:
arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability: 
> 2 years at 4oC.

Specific activity:
~ 100 U/mg (40oC, pH 8.0 on potato galactan).

Unit definition:
One Unit of galactanase activity is defined as the amount of enzyme required to release one μmole of galactose reducing-sugar equivalents per minute from potato galactan in sodium phosphate buffer (100 mM), pH 8.0 at 40oC. 

Specificity:
endo-hydrolysis of (1,4)-β-D-galactosidic linkages in galactans.

Applications:
Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping—Enzymatic digestion followed by chromatographic analysis.

Guan, J. & Li, S. P. (2010). Journal of Pharmaceutical and Biomedical Analysis, 51(3), 590-598.