endo-1,4-β-Xylanase M4 (Aspergillus niger

High purity endo-1,4-β-Xylanase M4 (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

CAZy Family: GH11
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

Highly purified. From Aspergillus niger. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,000 U/mL. 

Specific activity:
~ 80 U/mg (40oC, pH 4.5 on wheat arabinoxylan).

Stability: > 4 years at 4oC.

Product Code
8,000 Units

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endo-1,4-β-Xylanase M4 (Aspergillus niger)

CAZy Family: GH11
CAS: 9025-57-4

endo-1,4-beta-xylanase; 4-beta-D-xylan xylanohydrolase

In 3.2 M ammonium sulphate.

> 4 years at 4oC.

Specific activity:
~ 80 U/mg (40oC, pH 4.5 on wheat arabinoxylan).

Unit definition:
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

endo-hydrolysis of (1,4)-β-D-xylosidic linkages in xylans.

Applications in carbohydrate and biofuels research and in the food and feeds and paper pulping industries.

Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

A Comparison of Polysaccharide Substrates and Reducing Sugar Methods for the Measurement of endo-1,4-β-Xylanase.

McCleary, B. V. & McGeough, P. (2015). Appl. Biochem. Biotechnol., 177(5), 1152-1163.

Evaluation of the xylan breakdown potential of eight mesophilic endoxylanases.

Cuyvers, S., Dornez, E., Moers, K., Pollet, A., Delcour, J. A. & Courtin, C. M. (2011). Enzyme and Microbial Technology, 49(3), 305-311.

The use of Xylanases from different microbial origin in bread baking and their effects on bread qualities.

Al-Widyan, O., Khataibeh, M. H. & Abu-Alruz, K. (2008). Journal of Applied Sciences, 8(4), 672-676.

His374 of wheat endoxylanase inhibitor TAXI‐I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases.

Fierens, K., Gils, A., Sansen, S., Brijs, K., Courtin, C. M., Declerck, P. J., De Ranter, C. J., Gebruers, K., Rabijns, A., Robben, J., Van Campenhout, S., Volckaert, G. & Delcour, J. A. (2005). FEBS Journal, 272(22), 5872-5882.

Matrix solubilization and cell wall weakening by β-expansin (group‐1 allergen) from maize pollen.

Tabuchi, A., Li, L. C. & Cosgrove, D. J. (2011). The Plant Journal, 68(3), 546-559.

Changes in cell wall biomechanical properties in the xyloglucan-deficient xxt1/xxt2 mutant of Arabidopsis.

Park, Y. B. & Cosgrove, D. J. (2012). Plant Physiology, 158(1), 465-475.

New glycosidase substrates for droplet-based microfluidic screening.

Najah, M., Mayot, E., Mahendra-Wijaya, I. P., Griffiths, A. D., Ladame, S. & Drevelle, A. (2013). Analytical Chemistry, 85(20), 9807-9814.

Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction.

Loureiro, D. B., Romanini, D. & Tubio, G. (2016). Biocatalysis and Agricultural Biotechnology, 5, 204-210.

Fast automated online xylanase activity assay using HPAEC-PAD.

Cürten, C., Anders, N., Juchem, N., Ihling, N., Volkenborn, K., Knapp, A., Jaeger, K. E., Büchs, J. & Spiess, A. C. (2017). Analytical and bioanalytical chemistry, 410(1), 57-69.